响应面优化超声协同高压矩形脉冲电场提取黄花菜多糖工艺及其抗氧化活性研究

陆海勤; 宫晓丽; 李冬梅; 骆文静; 李毅花; 李文

doi:10.13386/j.issn1002-0306.2020.11.025

响应面优化超声协同高压矩形脉冲电场提取黄花菜多糖工艺及其抗氧化活性研究

Optimization of Ultrasound Combined with Rectangular High Pulsed Electrical Field Assisted Extraction of Polysaccharides from Hemerocallis citrina by Response Surface Methodology and Its Antioxidant Activity

摘要

摘要: 以黄花菜为原料,采用超声协同高压矩形脉冲电场提取黄花菜多糖,在单因素实验的基础上,利用响应面分析法优化超声协同高压矩形脉冲电场提取黄花菜多糖工艺,同时测定黄花菜粗多糖对·O₂^-、·OH以及DPPH·的清除能力。结果表明,超声协同高压矩形脉冲电场提取最佳工艺条件为:提取时间30 min,提取温度59 ℃,超声功率700 W,电场电压 14 kV,得到的黄花菜多糖得率为10.03%,此结果接近预测得率,表明提取工艺是可行的。体外抗氧化活性结果表明,黄花菜粗多糖有一定的清除能力,且与多糖浓度呈正相关,当黄花菜粗多糖浓度为1.0 mg/mL时对·O₂^-、·OH以及DPPH·的清除能率分别可达到66.93%、70.61%、49.28%。本研究为黄花菜多糖提供了一种新型的提取工艺。

Abstract: Based on the single factor experiment,response surface analysis was used to optimize the extraction process of polysaccharide from Hemerocallis citrina by ultrasonic synergy rectangular pulse high voltage electric field. Meanwhile,the scavenging abilities of polysaccharide from Hemerocallis citrina to ·O₂^-,·OH,DPPH· were determined.Results showed that the best process conditions of ultrasonic synergy rectangular pulse high voltage electric field were as follows:Extraction time 30 min,extraction temperature 59 ℃,ultrasound power 700 W,electrical voltage 14 kV,the yield of Hemerocallis citrina polysaccharide was 10.03%,which was close to the predicted yield,indicating the extraction process was feasible. The results of in vitro antioxidant activity showed that Hemerocallis citrina polysaccharide had certain scavenging ability on ·O₂^-,·OH,DPPH·,and positively related to the concentration of crude polysaccharide of Hemerocallis citrina,when the concentration of Hemerocallis citrina polysaccharide was 1 mg/mL,scavenging rates on three free radicals could reach 66.93%,70.61%,49.28% respectively. This study provided a new extraction technology for polysaccharides from Hemerocallis citrina.