还原型谷胱甘肽纳米脂质体的修饰及稳定性评价

魏竹君; 于少轩; 杨武; 王善钰; 宋元达

doi:10.13386/j.issn1002-0306.2020.11.005

还原型谷胱甘肽纳米脂质体的修饰及稳定性评价

Modification and Stability Evaluation of Reduced Glutathione Nano-liposomes

摘要

摘要: 本文通过薄膜-超声法制备了还原型谷胱甘肽的纳米脂质体(Lip),并用壳聚糖和海藻酸钠对Lip进行了双层修饰,然后测定了修饰前后Lip的贮藏稳定性和在胃肠道环境的消化特性,利用不同数学模型分析了谷胱甘肽在胃肠道的释放动力学。结果表明,海藻酸钠-壳聚糖双层修饰的Lip(AL-CH-Lip)形状为球形,平均粒径为(94.44±1.71) nm,电位为(-42.57±0.91) mV,包埋率为93.5%±0.16%。与未经修饰或单独壳聚糖修饰的Lip相比,AL-CH-Lip具有更高的贮藏稳定性和消化稳定性,更适合作为功能性添加剂应用于食品工业和医药等领域。

Abstract: In this work,reduced glutathione nano-liposomes(Lip)were prepared by thin-film ultrasonic method and double-modified with both chitosan and sodium alginate. Then the storage stability and digestion properties in gastrointestinal tract of Lip with or without modification were measured. Additionally,the release kinetics of glutathione in gastrointestinal tract was also analyzed through the usage of different models. The results showed that Lip modified with both sodium alginate and chitosan(AL-CH-Lip)had a spherical shape,an average size of(94.44±1.71) nm,and a Zeta potential of(-42.57±0.91) mV. Moreover,the loading efficiency of AL-CH-Lip was measured to be 93.5%±0.16%. Compared with unmodified or chitosan modified Lip,AL-CH-Lip had higher storage stability and digestion stability,and was more suitable for application in food industries and medical areas.