三七冻干粉及其活性组分对小鼠1型辅助性T细胞分化和活性的影响

张明菲; 范瑶婕; 杨培; 王祥; 周玲玲

doi:10.13386/j.issn1002-0306.2020.10.048

三七冻干粉及其活性组分对小鼠1型辅助性T细胞分化和活性的影响

Effect of Panax notoginseng Lyophilized Powder and Its Active Components on the Differentiation and Activation of Th1 Cell in Mice

摘要

摘要: 目的:观察三七冻干粉及其有效成分对小鼠CD4⁺T细胞向1型辅助性T(Th1)细胞分化的影响,探究三七作为药食同源药物改善机体免疫力的可能作用机制。方法:提取C57BL/6小鼠脾细胞并分选出CD4⁺T细胞进行培养;分为空白对照组,三七冻干粉低、中、高剂量组(160、320、640 μg/mL)和三七总皂苷低、中、高剂量组(5、10、20 μg/mL),加入对应药物48 h后,采用CCK-8法筛选三七冻干粉及三七总皂苷的最佳给药浓度并检测二者对细胞活力的影响,用酶联免疫吸附试验(ELISA)检测上清液中 IFN-γ的变化。结果:与对照组相比,三七冻干粉组和三七总皂苷组的细胞因子IFN-γ水平,IFN-γ mRNA和T-bet mRNA表达水平均显著降低(P<0.05)。结论:三七冻干粉及其活性成分均能抑制CD4⁺T细胞向Th1细胞分化。

Abstract: To observe the effect of Panax notoginseng lyophilized powder and Panax notoginseng saponins(PNS)on the differentiation of mouse CD4⁺T cells into Th1 cells,and to explore the potential mechanism of Panax notoginseng as a drug-synonym for improving immunity. Methods:Splenocytes from C57BL/6 mouse were selected and CD4⁺T cells were sorted for culture. Cells were divided into control group,Panax notoginseng lyophilized powder groups(160,320,640 μg/mL)and PNS groups(5,10,20 μg/mL),and cultured for 48 hours. The optimal concentration of Panax notoginseng lyophilized powder and PNS was screened by CCK-8 method. Effect of IFN-γ in supernatant was detected by enzyme-linked immunosorbent assay(ELISA). Relative expression levels of IFN-γ mRNA and T-bet mRNA were detected by q-PCR. Results:Compared with the control group,the expression level of IFN-γ,IFN-γ mRNA and T-bet mRNA in the Panax notoginseng lyophilized powder groups and PNS groups were significantly lower than control group(P<0.05). Conclusion:Panax notoginseng lyophilized powder and PNS could inhibit the differentiation and activity of Th1 cells.