Abstract: A method based on grinder-QuEChERS coupled with high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was developed for the determination of ten sulfonamides residues in animal-originated food. After mixing with EDTA,the sample was grinded,extracted with acetonitrile and Bond QuEChERS,purified by QuEChERS EMR-Lipid,analysed by HPLC-MS/MS. The separation of sulfonamides was carried out on a C₁₈ column with a gradient elution of 0.1% acetate and acetonitrile. The results showed that,the 10 kinds of sulfonamides had a good linear relationship in a certain concentration range with correlation coefficients(R²)greater than 0.99. The limits of detection(S/N=3)were 0.2 μg/kg,the limits of quantitation(S/N=10)were 0.5 μg/kg. The average recoveries of sulfonamides were 89.4%~111.0% and the relative standard deviations(RSDs)were 0.8%~4.9%. The method with a simple operation and a high recovery was suitable for the determination of 10 sulfonamides in animal-originated food. Furthermore,matrix-matched calibration curve could be used to compensate or eliminate the matrix effects in analysis of low concentration sulfonamides in swine,milk and egg samples.