Abstract: A method was established for the simultaneous determination of 44 kinds of residues in eggs by ultra-high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS). The sample was added with acetonitrile for protein deposition,then purified by techniques function composition of multi-walled carbon nanotubes,octadecyl bonded silica gel and ethylenediamine-N-propyl silane portfolio. It was gradient eluted and seperated with a 0.1% formic acid solution -0.1% formic acid methanol as mobile phase,measured under the monitoring mode that with electrospray ion source multiple reaction monitoring mode in positive and negative ions,and quantitated by using the matrix matching quantitative standard curve external standard method. The results showed that the 44 drugs had good linearity in the range of 0.1~200 ng/mL,R² was 0.9909~0.9999,the detection limit was 0.007~0.3571 μg/kg,the quantitative limit was 0.005~1.190 μg/kg,the recovery rate was 77.9%~114.5%,and the relative standard deviation was less than 10%(n=6). The method is simple,sensitive and apply to a high throughput screening of drug residues in eggs.