纤维素酶和果胶酶提取对甘草渣多糖抗氧化和抗肿瘤性能的影响

李菀; 胡月; 李光霞; 殷胜友; 姚惠文

doi:10.13386/j.issn1002-0306.2020.09.050

纤维素酶和果胶酶提取对甘草渣多糖抗氧化和抗肿瘤性能的影响

Effects of Cellulase and Pectinase Extraction on the Antioxidant and Antitumor Activities of Licorice Residue Polysaccharide

摘要

摘要: 目的:考察不同酶提取对甘草渣多糖抗氧化性和抗肿瘤性能的影响,为后续甘草资源的有效再利用提供依据。方法:选用纤维素酶和果胶酶,在实验的优化条件下分别提取甘草渣多糖,比较两种酶提取的甘草渣多糖的得率、红外光谱、抗氧化性和抗肿瘤性。结果:果胶酶和纤维素酶提取的多糖得率分别为8.43%和10.71%。红外光谱结果表明,两种酶提取的多糖均具有α-吡喃环糖环。抗氧化性实验结果表明,果胶酶和纤维素酶提取的多糖对DPPH自由基、羟基自由基、ABTS自由基清除的IC₅₀值分别为0.00243、0.305、0.0403 mg/mL和0.226、0.0238、0.0401 mg/mL。抗肿瘤结果表明,在0.00250~0.125 mg/mL浓度范围内,纤维素酶提取的多糖肿瘤细胞的抑制率高于果胶酶提取的多糖。结论:纤维素酶提取的多糖得率更高、对羟基自由基的清除能力和抗肿瘤性能更强,果胶酶提取的多糖对DPPH自由基的清除能力更强,两者对ABTS自由基的清除能力基本相同。

Abstract: Objective:To investigate the effects of different enzyme extraction methods on the antioxidant and antitumor properties of licorice residue polysaccharide and to provide evidence for the effective reuse of licorice. Methods:The licorice residue polysaccharides were extracted by cellulase and pectinase under optimized conditions respectively. The yield,IR spectra,antioxidant activity and antitumor activity were investigated and compared. Results:The yield of licorice residue polysaccharide extracted by pectinase and cellulase was 8.43% and 10.71%,respectively. The IR spectra confirmed the skeletal modes of the pyranose ring in licorice residue polysaccharide. The IC₅₀ values of DPPH,hydroxyl and ABTS radical scavenging activity of polysaccharide extracted by pectinase and cellulase were 0.00243,0.305,0.0403 mg/mL and 0.226,0.0238,0.0401 mg/mL,respectively. Antitumor results showed that the inhibition rate of cellulase extraction polysaccharide was higher than pectinase extraction polysaccharide in range of 0.00250~0.125 mg/mL. Conclusion:Cellulase extraction polysaccharide had high yield,better hydroxyl radical scavenging activity and antitumor property. Pectinase extraction polysaccharide had better DPPH free radical scavenging activity. The scavenging ability of ABTS free radical of them was same.