Abstract: A method for simultaneous determination of glucose,fructose,sucrose,maltose,lactose,erythritol,xylitol,sorbitol and maltitol by ion chromatography was developed. The pretreatment of samples and the selection of chromatographic column were optimized. The protein was precipitated by adjusting pH. The CarboPac PA1 column was used for separation. Sodium hydroxide-sodium acetate was used as the eluent for gradient elution and the detection was performed with a pulse amperometric detector. The results showed that the 9 kinds of sugars and sugar alcohols could be separated completely under the conditions of the method,and the linear relationship was good within the range of 0.1~10 μg/mL. The RSD of method repeatability was less than 3%,and the recovery rate was 96.54%~103.71%. According to the method,the sugar content of the sugar-free sample was lower than 0.5%,and the sugar contents of the low-sugar samples were lower than 5%. The method has the advantages of simple operation,high sensitivity and good separation,and can be used for quick and accurate determination of sugars and sugar alcohols in low-sugar and sugar-free food.