海洋链霉菌<i>Streptomyces</i> sp.MCCC 1A09830抗菌成分的分离与鉴定

刘象博; 柴树茂; 曹明明; 张改云; 冯治洋

doi:10.13386/j.issn1002-0306.2020.09.017

海洋链霉菌Streptomyces sp.MCCC 1A09830抗菌成分的分离与鉴定

Isolation and Identification of Antimicrobial Active Substance Produced by Marine Streptomyces sp. MCCC 1A09830

摘要

摘要: 目的:研究海洋链霉菌Streptomyces sp. MCCC 1A09830与其它菌种的亲缘关系,并分离纯化Streptomyces sp. MCCC 1A09830产生的抗菌成分,鉴定抗菌成分的结构。方法:通过构建系统发育树研究Streptomyces sp. MCCC 1A09830与其它菌种的亲缘关系;采用甲醇提取、硅胶柱层析、高效液相色谱法等分离技术纯化抗菌成分,鉴定抗菌成分的结构。结果:Streptomyces sp. MCCC 1A09830与Streptomyces sp. NEAE-126存在很近的亲缘关系;Streptomyces sp. MCCC 1A09830产生的抗菌成分得到了纯化,获得了化合物1。化合物1经高分辨质谱(High Resolution Mass Spectrometry,HRMS)、一维及二维核磁共振(Nuclear Magnetic Resonance,NMR)分析鉴定为Borrelidin A(疏螺旋体素)。结论:从Streptomyces sp. MCCC 1A09830的发酵液中分离得到1种抗菌成分,丰富了Borrelidin的生产菌库,为海洋菌株活性产物的分离提供了支持。

Abstract: Objective:This study investigated the relationship between marine Streptomyces sp. MCCC 1A09830 and other strains,and isolated and purified the antimicrobial active substance which was produced by Streptomyces sp. MCCC 1A09830,and identified the structure of antimicrobial active substance. Method:The relationship between Streptomyces sp. MCCC 1A09830 and other strains was investigated by constructing phylogenetic tree. Methanol extraction,silica gel column chromatography and high performance liquid chromatography were used to isolate and purify the the antimicrobial active substances,and the structure of antimicrobial active substance was identified. Results:There was a close relationship between Streptomyces sp. MCCC 1A09830 and Streptomyces sp. NEAE-126. The antimicrobial active substance produced by Streptomyces sp. MCCC 1A09830 was purified and compound 1was obtained. The structure of compound 1 was identified as Borrelidin A by Mass Spectrometry and Nuclear Magnetic Resonance. Conclusion:One antimicrobial active substance was isolated from fermentation broth of Streptomyces sp. MCCC 1A09830,which enriched the strain that produce Borrelidin and provide support for isolation of active substances produced by marine strains.