Abstract: The lily polysaccharide was purified and explored for its regulation of intestinal dysregulated mice. The purification effects of four macroporous resins AB-8,D315,D101 and HPD-100 on lily polysaccharides were compared by the yields,decolorization rates and polysaccharide contents. The experiment was divided into blank group(saline 10 mg/kg),model group(lincomycin hydrochloride 10 mg/kg),positive group(lizhu intestine 10 mg/kg),and the low,medium and high(50,100,200 mg/kg)dose groups of lily polysaccharide. The mouse ileal tissue SIgA,plasma LPS,serum inflammatory factor IL-6,the contents of TNF-α and the number of microflora were used as indicators to evaluate the regulation effect of purified lily polysaccharide on intestinal flora imbalance mice. The results showed that the yields,decolorization rates and polysaccharide content of lily polysaccharide purified by D315 macroporous resin were the highest with 40.82%±1.28%,35.11%±1.12% and 82.56%±1.18%,respectively. The results of pharmacological experiments showed that the high dose group of lily polysaccharide had the best effect. Compared with the model group,the LPS contents,serum inflammatory factors IL-6 and TNF-α levels in the high dose group were significantly(P<0.01)decreased,and the ileal tissue SIgA contents was significantly increased(P<0.01),the number of Enterobacteriaceae and Enterococcus were significantly reduced(P<0.05),while the number of Lactobacillus and Bifidobacteria were increased(P<0.05). It indicated that lily polysaccharide had a regulatory effect on the intestinal flora disorder in mice.