Abstract: An indirect competitive ELISA for triamcinolone acetonide(TCA)was developed to monitor TCA abuse. To obtain the TCA complete antigen,TCA hapten and keyhole limpet hemocyanin(KLH)were coupled by using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride(EDC)and N-Hydroxysuccinimide(NHS). The homologous detection antigen TCA-bovine serum albumin(TCA-BSA)and heterologous detection antigen Dexamethasone-BSA(DEX-BSA)were synthesized in the same way. After immunization and cell fusion,one strain of hybridoma cell line that could steadily secrete anti-TCA monoclonal antibody was prepared and its antibody(ascites)was prepared and purified. By using the antibody and two different detection antigens,both homologous and heterologous strategy indirect competitive enzyme linked immunosorbent assay(ic-ELISA)standard curve for detection of TCA was built,recoveries of TCA added to liquid milk was detected by the heterologous strategy. The results showed that the IC₅₀ of homologous strategy ic-ELISA was 5.4 ng/mL,the IC₅₀ of heterologous strategy ic-ELISA was 0.53 ng/mL.The method did not cross reacted with other glucocorticoids except halcinonide,budesonide and amcinonide. The recovery rates of TCA added to animal tissues ranged from 75% to 90% using 20% methanol/water. The method can be applied in TCA fast screening in animal tissue.