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中国精品科技期刊2020
李晗, 杨宗玲, 毕永雪, 张蕊, 徐玉巧, 范方宇. 超声辅助酶法提取西番莲果皮可溶性膳食纤维及理化性质[J]. 华体会体育, 2020, 41(7): 161-165,172. DOI: 10.13386/j.issn1002-0306.2020.07.027
引用本文: 李晗, 杨宗玲, 毕永雪, 张蕊, 徐玉巧, 范方宇. 超声辅助酶法提取西番莲果皮可溶性膳食纤维及理化性质[J]. 华体会体育, 2020, 41(7): 161-165,172. DOI: 10.13386/j.issn1002-0306.2020.07.027
LI Han, YANG Zong-ling, BI Yong-xue, ZHANG Rui, XU Yu-qiao, FAN Fang-yu. Extraction of Soluble Dietary Fiber from Passiflora edulis Peel by Ultrasonic Assisted Enzymatic Method and Its Physicochemical Properties[J]. Science and Technology of Food Industry, 2020, 41(7): 161-165,172. DOI: 10.13386/j.issn1002-0306.2020.07.027
Citation: LI Han, YANG Zong-ling, BI Yong-xue, ZHANG Rui, XU Yu-qiao, FAN Fang-yu. Extraction of Soluble Dietary Fiber from Passiflora edulis Peel by Ultrasonic Assisted Enzymatic Method and Its Physicochemical Properties[J]. Science and Technology of Food Industry, 2020, 41(7): 161-165,172. DOI: 10.13386/j.issn1002-0306.2020.07.027

超声辅助酶法提取西番莲果皮可溶性膳食纤维及理化性质

Extraction of Soluble Dietary Fiber from Passiflora edulis Peel by Ultrasonic Assisted Enzymatic Method and Its Physicochemical Properties

  • 摘要: 为提高西番莲果皮废弃物的利用率,以西番莲皮可溶性膳食纤维(Passion Peel Soluble Dietary Fiber,PSDF)得率为评价指标,通过单因素实验分析料液比、酶解温度、超声功率、混合酶量(淀粉酶和木瓜蛋白酶比例为1:1)对PSDF得率的影响,结合正交试验优化提取工艺,并对西番莲果皮粉(Passion Peel Flour,PPF)和PSDF的理化性质进行分析。结果表明,超声辅助酶法提取PSDF最佳工艺条件为:料液比1:26 g/mL、酶解温度70℃、超声功率250 W、混合酶量0.6%,PSDF得率为14.82%。与PPF相比,PSDF溶胀性和持水力极显著增加了52.29%、19.66%(P<0.01);堆积密度显著下降了24.18%(P<0.05);饱和脂肪酸和不饱和脂肪酸结合力分别增加了1.97%、8.4%(P>0.05);模拟胃环境(pH=2.0)和肠道环境(pH=7.0)的胆固醇吸附能力分别显著增加了16.15%、10.47%(P<0.05)。红外光谱表明,PPF和PSDF均具有典型的多糖特征吸收峰。

     

    Abstract: In order to improve the utilization rate of Passiflora edulis peel wastes,this study using Passiflora edulis peel as raw material,the effects of solid/solvent ratio,enzymatic hydrolysis temperature,ultrasonic power and mixed enzyme content(the ratio of α-amylase to papain was 1:1)on the the yield of passion peel soluble dietary fiber(PSDF)were investigated parameters through single factor testusing. The extraction process was optimized ocombined with orthogonal array. The physicochemical properties of passion peel flour(PPF)and PSDF were evaluated. The results indicated that the optimum conditions of ultrasonic assisted enzyme extraction of PSDF were as follows:Solid/solvent ratio of 1:26 g/mL,hydrolysis temperature of 70℃,ultrasonic power of 250 W,and mixed enzyme content of 0.6%. Under the optimal extraction conditions,the yield of PSDF was 14.82%. Compared with PPF,the swelling property and WHC of PSDF were significantly increased by 52.29% and 19.66%(P<0.01),respectively. The apparent density was decreased by 24.18%(P<0.05). The binding capacity of saturated fatty acid and unsaturated fatty acid were increased by 1.97% and 8.4%(P>0.05),respectively. And the adsorption capacity of cholesterol in simulated gastric environment(pH=2.0)and intestinal environment(pH=7.0)were increased by 16.15% and 10.47%(P<0.05),respectively. The characteristic absorption peaks of two kinds of samples were characterized by infrared spectrum,these two samples were indicative of the presence of the typical structure of polysaccharides.

     

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