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中国精品科技期刊2020
周晏琳, 刘洋, 李亚烜, 闫丽新, 田元勇, 刘俊荣. 虾夷扇贝抗氧化酶SOD和CAT与活品贮藏稳定性的关联[J]. 华体会体育, 2020, 41(6): 254-258,271. DOI: 10.13386/j.issn1002-0306.2020.06.043
引用本文: 周晏琳, 刘洋, 李亚烜, 闫丽新, 田元勇, 刘俊荣. 虾夷扇贝抗氧化酶SOD和CAT与活品贮藏稳定性的关联[J]. 华体会体育, 2020, 41(6): 254-258,271. DOI: 10.13386/j.issn1002-0306.2020.06.043
ZHOU Yan-lin, LIU Yang, LI Ya-xuan, YAN Li-xin, TIAN Yuan-yong, LIU Jun-rong. Correlation of Antioxidant Enzyme SOD and CAT to Live Storage Stability of Yesso scallop(Patinopecten yessoensis)[J]. Science and Technology of Food Industry, 2020, 41(6): 254-258,271. DOI: 10.13386/j.issn1002-0306.2020.06.043
Citation: ZHOU Yan-lin, LIU Yang, LI Ya-xuan, YAN Li-xin, TIAN Yuan-yong, LIU Jun-rong. Correlation of Antioxidant Enzyme SOD and CAT to Live Storage Stability of Yesso scallop(Patinopecten yessoensis)[J]. Science and Technology of Food Industry, 2020, 41(6): 254-258,271. DOI: 10.13386/j.issn1002-0306.2020.06.043

虾夷扇贝抗氧化酶SOD和CAT与活品贮藏稳定性的关联

Correlation of Antioxidant Enzyme SOD and CAT to Live Storage Stability of Yesso scallop(Patinopecten yessoensis)

  • 摘要: 为探究贝类抗氧化体系与其活品贮藏稳定性之间的关系,以虾夷扇贝(Patinopecten yessoensis)为研究对象,对抗氧化酶在各个软体组织部位中的分布和贮藏期间的变化进行分析。原料活品扇贝为当日手工潜水采集,分组进行冷却干露(4℃)贮藏,分别于0、1、3 d对外套膜、性腺、横纹肌、鳃及内脏进行分离取样,并对干藏过程中各组织的超氧化物歧化酶(SOD)和过氧化氢酶(CAT),闭壳肌pH、糖原含量、ATP及其关联化合物,以及扇贝失重率等系列指标的变化进行分析。结果表明:虾夷扇贝各组织均检测到SOD和CAT的活性,横纹肌中的SOD活性较高,鳃和内脏组织中的SOD和CAT与其他软体组织相比,酶活更为显著(P<0.05);干藏初期1 d为平稳期,SOD、CAT、ATP、pH保持平稳;1~3 d为波动期,各软体组织SOD活性显著上升(P<0.05),CAT、ATP及其关联物含量、pH以及糖原含量等显著变化(P<0.05)。从ATP及其关联物含量来看,3 d冷却干藏后虽略降低但活力保持较好,除了ADP、AMP及AdR有检出,ATP的进一步降解产物未检出;糖原含量从初始21.5 mg/g降至19 mg/g左右;pH从7降至6.7左右。同时,干藏期间贝体失重率呈现上升趋势,至第3 d失重率为8%。综上,冷却干藏期间扇贝活力状态具有阶段性差异,扇贝各软体组织抗氧化酶SOD和CAT活性呈显著变化(P<0.05),其中SOD尤为明显。

     

    Abstract: To explore the quality changing mechanism in metabolic suppression of post-harvest live scallop,superoxide dismutase(SOD)and catalase(CAT)in different soft tissues were taken as indicators of quality change during live dry-storage of scallop(Patinopecten yessoensis)for 3 days. The raw live scallops were hand-dived on the treatment day. The treatment was carried out at 4℃ and soft tissues were separated into mantle,gonad,striated muscle,gill and visceral mass. The SOD and CAT activities were detected in all tissues,indicating that the SOD activity of striated muscle,gill and visceral mass was high and the CAT activity of gill and visceral mass was higher than other tissues. SOD,CAT,ATP and ATP-related compounds were maintained during the initial stage of 1 d. From 1 to 3 days was the fluctuation period,the SOD activity of each soft tissue increased significantly(P<0.05),and the contents of CAT,ATP and related substances,pH,and glycogen contents changed significantly(P<0.05). From the contents of ATP and its related substances,it was slightly reduced after during chilling dry storage in 3 days,and its vitality remained good,except for ADP,AMP and AdR,no degradation products of ATP were detected. The contents of glycogen decreased from 21.5 mg/g to 19 mg/g and pH declined from 7 to 6.7. Besides,the mass loss rate of scallops increased significantly to 8% by the 3 rd day. These findings suggest that the post-harvest quality-changing mechanisms of live scallops had different in successive stages. Antioxidant enzyme SOD and CAT activities in scallop soft tissues showed significant changes(P<0.05),and SOD was particularly significant.

     

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