双层平板直接定性定量法检测海产品中副溶血性弧菌

周霞霞; 邢家溧; 傅晓; 王绍辉; 承海; 章海通; 吴莹莹; 陈灿灿

doi:10.13386/j.issn1002-0306.2020.06.038

双层平板直接定性定量法检测海产品中副溶血性弧菌

Direct Qualitative and Quantitative Detection of Vibrio parahaemolyticus in Seafood by Double Plate Method

摘要

摘要: 目的:建立一种直接定量定性检测海产品中副溶血性弧菌的方法。方法:参照国标菌落计数方法,筛选具备计数和显示副溶血性弧菌典型菌落颜色功能的培养基,将人工染菌样品分别置于50、4和-18℃温度下并分别进行测试。结果:在NaCl NA双层平板上副溶血性弧菌菌落呈淡紫色,在人工染菌样品实验中与NaCl NA平板计数无显著差异;NaCl NA双层平板直接定量检测4℃冷藏处理和-18℃冷冻的样品中副溶血性弧菌浓度分别是对照组的68.9%和21.7%。结论:NaCl NA双层平板法具备操作简便、检验周期短、结果稳定、灵敏度高等特点,适合应用于-18~50℃温度环境下放置的海产品中副溶血性弧菌直接定性定量检测,能如实反映样品受污染的情况。

Abstract: Objective:To establish a direct quantitative and qualitative detection method of Vibrio parahaemolyticus in seafood. Method:According to colony counting method of GB,the culture to count and recognize typical colony color from V.parahaemolyticus was screened. Artificially contaminated samples were tested at 50、4 and -18℃. Results:In NaCl NA double-layer agar,the number of lavender colonies from V.parahaemolyticus was no significant difference from that on NaCl NA plate in artificial bacterial contamination test. Under 4 and-18℃,the concentrations of V.parahaemolyticus of samples were 68.9% and 21.7% compared with the control group detected by NaCl NA double-layer agar direct quantitative test. Conclusion:NaCl NA double-layer agar had the advantages of simplicity,rapidity,stable result and highly sensitivity,suitable for the direct quantitative and qualitative detection of V. parahaemolyticus in seafood under-18~50℃ environment and truthfully reflecting the contamination of samples.