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中国精品科技期刊2020
曹冠华, 张雪, 王希付, 熊记, 王杰, 李晓岗, 肖进宝, 赵荣华, 贺森. 阳春砂仁生品与盐制砂仁不同溶剂萃取物抑菌效果比较[J]. 华体会体育, 2020, 41(6): 82-87. DOI: 10.13386/j.issn1002-0306.2020.06.014
引用本文: 曹冠华, 张雪, 王希付, 熊记, 王杰, 李晓岗, 肖进宝, 赵荣华, 贺森. 阳春砂仁生品与盐制砂仁不同溶剂萃取物抑菌效果比较[J]. 华体会体育, 2020, 41(6): 82-87. DOI: 10.13386/j.issn1002-0306.2020.06.014
CAO Guan-hua, ZHANG Xue, WANG Xi-fu, XIONG Ji, WANG Jie, LI Xiao-gang, XIAO Jin-bao, ZHAO Rong-hua, HE Sen. Comparison of Bacteriostatic Effects of Different Extracts from Fructus amomi and Its Salt-processed Products[J]. Science and Technology of Food Industry, 2020, 41(6): 82-87. DOI: 10.13386/j.issn1002-0306.2020.06.014
Citation: CAO Guan-hua, ZHANG Xue, WANG Xi-fu, XIONG Ji, WANG Jie, LI Xiao-gang, XIAO Jin-bao, ZHAO Rong-hua, HE Sen. Comparison of Bacteriostatic Effects of Different Extracts from Fructus amomi and Its Salt-processed Products[J]. Science and Technology of Food Industry, 2020, 41(6): 82-87. DOI: 10.13386/j.issn1002-0306.2020.06.014

阳春砂仁生品与盐制砂仁不同溶剂萃取物抑菌效果比较

Comparison of Bacteriostatic Effects of Different Extracts from Fructus amomi and Its Salt-processed Products

  • 摘要: 以阳春砂仁及其炮制品(盐制砂仁)为原料,经打粉过筛,不同浓度乙醇超声浸提,石油醚、乙酸乙酯、正丁醇分级萃取,制得不同溶剂砂仁粗提物,以大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌为供试菌,通过测定抑菌圈直径与最小抑菌浓度,比较砂仁生品与盐制砂仁乙醇浸提物及不同溶剂萃取物的抑菌效果。结果表明盐制砂仁95%乙醇浸提物抑菌效果优于75%和65%乙醇浸提物,且不同浓度乙醇浸提物对3种供试菌的抑菌效果均为砂仁生品优于盐制砂仁;不同溶剂萃取物中,砂仁生品和盐制砂仁的石油醚萃取物的抑菌效果最为显著,尤以对枯草芽孢杆菌的抑菌效果最好,抑菌圈直径分别为14.83、12.11 mm,最小抑菌浓度(MIC)分别为0.23、0.44 mg/mL。砂仁生品不同溶剂萃取物对3种供试菌的抑菌效果均优于盐制砂仁。

     

    Abstract: The crude extracts of Fructus amomi and its salt-processed products were obtained by beating into powder and sieveing,ultrasonic extraction assisted by different concentrations of ethanol and then fractional extraction with petroleum ether,ethyl acetate or n-butanol. The crude extracts were used to test bacteriostatic effect through diameter of bacteriostatic circle and minimum bacteriostatic concentration against three strains(E. coli,S. aureus and B. subtilis). The results showed that 95% ethanol extracts of salt-processed Fructus amomi had better bacteriostatic effect than 75% and 65% ethanol extracts. In addition,the bacteriostatic effect of different ethanol extracts of Fructus amomi were better than that of salt-processed Fructus amomi against the three test strains. Among the different solvent extracts,petroleum ether extracts of Fructus amomi and salt-processed products had the best bacteriostatic effect,particular in B. subtilis,and the inhibition zone diameters were 14.83 and 12.11 mm,respectively. Correspondingly,the minimum inhibitory concentrations(MIC)of petroleum ether extracts of Fructus amomi and salt-processed products on B. subtilis were 0.23 and 0.44 mg/mL,respectively. The bacteriostatic effect of different extracts of Fructus amomi is better than that of salt-processed Fructus amomi on the three strains.

     

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