壳寡糖复合固体饮料对2型糖尿病小鼠糖脂代谢紊乱的调节作用分析

姜雅杰; 何博韬; 王畅; 席茂盛; 罗学刚; 陈列欢

doi:10.13386/j.issn1002-0306.2020.05.044

壳寡糖复合固体饮料对2型糖尿病小鼠糖脂代谢紊乱的调节作用分析

Studies on the Regulation Effects of Chitooligosaccharides Compound Solid Beverage on Glyeolipid Metabolism Disorder of Type 2 Diabetes Mice

摘要

摘要: 为探讨壳寡糖复合固体饮料对T2DM小鼠糖脂代谢的影响,70只小鼠被随机分为正常对照组(NFD)、模型组(T2DM)、阳性对照组(T2DM+Acar)、壳寡糖组(T2DM+COS)、壳糖平低剂量组(T2DM+LKTP)、壳糖平中剂量组(T2DM+MKTP)、壳糖平高剂量组(T2DM+HKTP),依照实验剂量灌胃4周后,测定各组小鼠血清TC、TG、LDL-C、HDL-C及ALT、AST等相关指标。结果显示:壳糖平能够显著提高T2DM小鼠体重和降低空腹血糖(P<0.05),使血清中TC、TG及LDL-C、AI、心脏和肝脏指数、ALT、AST显著降低(P<0.05)。同时,肝脏和肾脏病理切片观察表明,壳糖平能明显改善肝肾细胞脂肪变性。小鼠肝脏组织蛋白和mRNA水平结果显示:与模型组相比,壳糖平可显著下调SREBP-2、HMGCR、G6pase、及PEPCK基因表达,并上调CYP7A1、LDLR基因(P<0.05)表达。这些研究表明,壳糖平具有辅助降低T2DM小鼠血脂及稳定血糖的作用。

Abstract: To investigate the effect of chitooligosaccharides compound solid beverage on glucose and lipid metabolism in mice with type 2 diabetes(T2DM),seventy mice were randomly divided into normal control group(NFD),model group(T2DM),positive control group(T2DM+Acar),chitooligosaccharides group(T2DM+COS),and Ke Tang Ping groups with low(T2DM+LKTP),middle(T2DM+MKTP),high dose(T2DM+HKTP). After being treated for 4 weeks,serum levels of TC,TG,LDL-C,HDL-C,ALT and AST were detected. The results showed that Ke Tang Ping could significantly increase the body weight of T2DM,decrease serum glucose,TC,TG and LDL-C,AI,liver and cardiac index,ALT and AST(P<0.05).At the same time,it could also markedly inhibit the pathological damage of liver and kidney. Protein and mRNA levels in mouse liver tissue were determined. Finally,compared with the T2DM group,some key enzymes in glycolipid metabolism were regulated by Ke Tang Ping,SREBP-2,HMGCR,G6pase and PEPCK were significantly down-regulated,whereas CYP7A1 and LDLR were up-regulated after the treatment of Ke Tang Ping(P<0.05). Taken together,Ke Tang Ping could reduce blood lipid and blood glucose in T2DM mice.