Abstract: To understand the differences of volatile content and main aroma between different distillation fractions of honeysuckle flower,the volatile components of 7 different distillation fractions of honeysuckle flower were analyzed by headspace-solid phase microextraction-gas chromatography-mass spectrometry. 62 kinds of volatile compounds were identified,mainly including alcohols,aldehydes,terpenoids,ketones and esters. Semi-quantitative results showed that the total content of volatile components in the distillate gradually decreased from 214.49 to 12.21 μg/g as distillation proceeded. The volatile content in the first distillation fraction was about 4 to 17 times that of other distillation fractions. Moreover,the proportions of alcohols,aldehydes,terpenoids,ketones and esters in different distillation fractions were different. The alcohols gradually decreased from 38.78% to about 25%,while the aldehydes increased from 32.60% to about 45%. β-Ionone,(E,E)-2,4-nonadienal,(E,E)-2,4-decadienal,(E)-2-nonenal,1-octene-3-ol,geraniol,citronellol,linalool,(E)-2-hexenal,geranyl acetone,n-hexanal,phenylacetaldehyde and n-octanol had a high odor activity value(greater than 10)in all the distillation fractions. They contributed a lot to the main aroma of the honeysuckle flower distillation fractions. The results of systematic cluster analysis indicated that the first,second and third distillation fractions could be each classified into one class,and the fourth to seventh distillation fractions could be classified into one class. In summary,the differences of volatile content and main aroma between different distillation fractions of honeysuckle flower were obviously. The results can provide a theoretical basis and reference for the improvement of the distillation process of the honeysuckle flower.