核桃低聚肽对亚健康大鼠氧化应激和炎症反应的影响

刘睿; 康家伟; 珠娜; 张亭; 王天星; 杜倩; 张黎; 李勇

doi:10.13386/j.issn1002-0306.2020.04.054

核桃低聚肽对亚健康大鼠氧化应激和炎症反应的影响

Effect of Walnut Oligopeptides on Inhibiting Oxidative Stress and Inflammation of Sub-health Rats

摘要

摘要: 目的:研究核桃低聚肽(walnut oligopeptides,WOPs)对亚健康大鼠的保护作用及其机制。方法:50只健康雄性SD大鼠随机分成5组:正常对照组、模型对照组和3个核桃低聚肽干预组(剂量分别为220、440、880 mg/kg·bw)。模型对照组和3个核桃低聚肽剂量组大鼠连续睡眠剥夺5 d后,进行负重力竭游泳实验,记录力竭游泳时间,并测定大鼠血清生化指标、氧化应激和脂质过氧化指标及细胞因子水平。结果:与模型对照组相比,核桃低聚肽组大鼠力竭游泳时间明显延长,血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)水平显著降低,超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)活性显著升高(P<0.05或P<0.01)。结论:桃低聚肽可以防治亚健康状态对机体的伤害,其机制可能与提高机体抗氧化水平,降低炎症因子水平相关。

Abstract: Objective:This study aimed to evaluate the effects of walnut oligopeptides(WOPs)on sub-health rats and explore the possible underlying mechanism. Methods:Fifty male SD rats were randomly divided into five experimental groups including normal control group,model control group,and three WOPs intervention groups(220、440、880 mg/kg·bw). The rats in model control group and WOPs groups were sleep deprivation for five consecutive days,and the exhausted swimming time,serum biochemical indicators,inflammatory cytokines and expression of oxidative stress markers were measured. Results:It was observed that,compared with the model control group,WOPs could significantly prolong the swimming time,decrease the levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α)and interleukin-1β(TL-1β),and increase the activities of superoxide dismutase(SOD)and reduce glutathione(GSH)(P<0.05 or P<0.01). Conclusion:These findings suggested that WOPs have a significant protective effect on sub-health atatus,and the mechanism possibly mediated by the partial inhibition of oxidative stress and inflammatory cytokine level.