沙棘多糖对D-半乳糖致衰老小鼠的抗氧化作用

包晓玮; 李建瑛; 任薇; 魏晨业; 曾兰君; 张亚涛

doi:10.13386/j.issn1002-0306.2020.04.050

沙棘多糖对D-半乳糖致衰老小鼠的抗氧化作用

Antioxidant Effects of Hippophae rhamnoides Polysaccharide on Aging Mouse Induced by D-galactose

摘要

摘要: 评价PBP(polysaccharide from Hippophae rhamnoides,PBP)对D-半乳糖致亚急性衰老模型小鼠的抗氧化作用。小鼠分为空白组、衰老模型组、阳性组(V_E 170 mg/kg)、PBP低(100 mg/kg)、中(200 mg/kg)、高(400 mg/kg)剂量组,连续灌胃30 d,测定其脏器指数、肝、脑组织及血清的SOD、GSH-Px活性及MDA含量;HE染色观察各组动物肝脏及脑组织细胞及结构变化。结果表明,与模型组相比,PBP高剂量及V_E组小鼠体质量增长幅度最大,PBP高、中及V_E组的脑、心脏及脾脏指数与模型组相比均显著提高(P<0.05,P<0.01);PBP高、中剂量组中肝、脑及血清中的GSH-Px均极显著高于模型组(P<0.01),PBP高剂量中肝、脑及血清中的SOD均极显著高于模型组(P<0.01),PBP高剂量及V_E组中肝、脑及血清中的MDA均极显著低于模型组(P<0.01)。组织切片HE染色发现PBP对衰老导致小鼠肝、脑组织细胞及结构损伤具有保护作用。PBP对D-半乳糖致亚急性衰老模型小鼠的保护作用可能与其具有抗氧化作用有关。

Abstract: To evaluate the antioxidant effect of polysaccharide from Hippophae rhamnoides(PBP)on subacute aging mice induced by D-galactose,the mice were divided into blank group,aging model group,positive group(V_E170 mg/kg),low(100 mg/kg),middle(200 mg/kg)and high(400 mg/kg)dose group of PBP,and fed continuous gavage for 30 d. The viscera indexes,activities of SOD,GSH-Px and MDA content in liver,brain and serum of each group were determined. The changes of liver and brain cells and structures were observed by HE staining.The results showed that,compared with the model group,the body mass of mice biggest increase were high dose PBP group and V_E group,and the brain,heart and spleen index of mice in high,middle PBP and V_E group increased significantly compared with the model group(P<0.05,P<0.01).GSH-Px in liver,brain and serum of high,medium PBP group were higher than those in model group(P<0.01),SOD activity in liver,brain and serum of high PBP group were higher than those in model group(P<0.01). MDA content in liver,brain and serum of high dose and V_E group were lower than those in model group(P<0.01).HE staining results of tissue sections showed that PBP had protective effect on liver,brain tissue cells and structure damage induced by aging in mice. The protective effect of PBP on D-galactose induced subacute aging mice may be related to its antioxidant effect.