蛋白修饰植物甾醇脂质体的制备及体外消化研究

王雪晖; 程铭; 焦文佳; 陶冶; 夏廉臣; 王春维; 祝爱侠

doi:10.13386/j.issn1002-0306.2020.02.010

蛋白修饰植物甾醇脂质体的制备及体外消化研究

Preparation and in Vitro Digestion of Protein Modified Phytosterol Liposomes

摘要

摘要: 本研究以植物甾醇(PS)替代胆固醇与卵磷脂(PC)为原料,以粒径、多分散系数PdI、电位和包封率为指标,采用乙醇注入法制备蛋白修饰的植物甾醇脂质体(SPI-LS),通过单因素优化脂质体制备工艺,研究了SPI-LS的复溶性及其在水中的溶解度,并考察了植物甾醇包埋前后体外消化生物活性。结果表明,最佳制备工艺为:PS:PC为1:4,乙醇体积比为25%,蛋白含量为1%;脂质体复溶后外观与冻干前无显著差异,溶解度可达1.971 mg/mL;经3 h体外胃肠消化后,SPI-LS中植物甾醇的生物活性仍可保持在50%以上,是未经包埋植物甾醇的三倍之多。故优化制备条件得到的SPI-LS复溶性良好,并能有效保持植物甾醇的生物活性。

Abstract: In this study,phytosterol(PS)and phosphatidylcholine(PC)were used as materials to prepare protein-modified phytosterol liposomes(SPI-LS)by ethanol injection method. Particle size,PdI,Zata potential,and the embedding rate were used to evaluate the technology conditions of preparation liposome by single factor optimization. The solubility of SPI-LS in water was observed. The bioactivity of digestion in vitro before and after PS embedding were investigated. The results showed that the optimum and technological condition were as follows:The ratio of PS to PC 1:4,ethanol volume ratio 25%,and protein composed 1%. There was no significant difference in the appearances of liposomes after solubilization and before lyophilization,and the solubility of PS was up to 1.971 mg/mL. After 3 h in vitro gastrointestinal digestion,the bioaccessibility of PS in SPI-LS could be maintained more than 50%,which was three times higher than that of free PS.Therefore,the most optimal SPI-LS had good solubility,and could effectively maintain the biological activity of phytosterols.