Abstract: Objective:To study the effects of different temperatures on the rheological properties of donkey milk. Method:Selecting test temperature 4,20,36,50℃,the fluidity,thixotropy,dynamic viscoelasticity of donkey milk were determined,and the rheological model was used for fitting analysis. Result:Donkey milk was a non-Newtonian fluid and showed shear-thinning behavior and its flow curve obeyed Carreau model,the viscosity decreased and the hysteresis loop area formed in the thixotropic test became smaller with the temperature increasing;In the viscoelasticity test,the storage modulus G' at 4℃ showed a maximum value and the storage modulus G' and the loss modulus G″ were highly dependent on temperature. The viscosity of the donkey milk decreased with the increase of temperature at the temperature range from 4 to 50℃.Conclusion:The rheology of the donkey milk was highly depending on temperature.