Abstract: Taking the sika deer tail as research object,ultrasonic water extraction of protein technology and water enzymatic extraction of peptides were optimized by orthogonal test. The proliferation activity of TM₃ cells was compared between the two optimally obtained proteins. The results showed that the optimal ultrasonic water extraction of protein process were as follows:The ratio of material to liquid was 1:5 g/mL,the ultrasonic power was 200 W,the extraction time was 1 h,and the extraction temperature was 40 ℃,the content of protein was 60.72%±0.56% during the condition. The best protease of water enzymatic extraction of peptides was trypsin. The optimal conditions for enzymatic hydrolysis were pH8,enzyme amount addition 1500 U/g,enzymatic hydrolysis for 4 h at 50 ℃,and degree of hydrolysis was 22.85%±0.35% during the condition. The enzymatic extraction of deer tail polypeptide on the proliferation of TM₃ was superior to that of ultrasonic water extracting deer tail protein.