腐植酸钠对植物乳杆菌生长、抑菌和黏附能力的影响

刘娜; 安晓萍; 张倩茹; 王园; 王瑞芳; 齐景伟

doi:10.13386/j.issn1002-0306.2019.22.021

腐植酸钠对植物乳杆菌生长、抑菌和黏附能力的影响

Effects of Sodium Humate on the Growth,Bacteriostasis and Adhesion Ability of Lactobacillus plantarum

摘要

摘要: 本试验旨在研究添加0.5%~8.0%的腐植酸钠对植物乳杆菌生长、抑制大肠杆菌和黏附能力的影响,通过对大肠杆菌的抑菌率、自凝集率、表面疏水率和与大肠杆菌的共凝集率进行研究。结果表明,添加0.5%~1.0%腐植酸钠可使植物乳杆菌的抑菌率提高19.18%~75.54%;添加4.0%~8.0%腐植酸钠在2~6 h可显著(P<0.05)提高植物乳杆菌的自凝集率和与大肠杆菌的共凝集率;添加2.0%~4.0%腐植酸钠可使植物乳杆菌的表面疏水率提高112.5%~128.57%。综上所述,选择合适浓度的腐植酸钠可提高植物乳杆菌的抑菌和黏附能力。

Abstract: The effects of sodium humate(0.5%~8.0%)on the growth,bacteriostatic and adhesion ability of Lactobacillus plantarum were studied. The Lactobacillus plantarum bacteriostasis rate to Escherichia coli,self-agglutination rate,surface hydrophobicity and co-agglutination rate with Escherichia coli were determined. The results showed that adding 0.5%~1.0% sodium humate could improve the antibacterial rate of Lactobacillus plantarum for 19.18%~75.54%. Adding 4.0%~8.0% sodium humate could improve the auto-aggregation rate of Lactobacillus plantarum and the co-agglutination rate with Escherichia coli at 2~6 h. Adding 2.0%~4.0% sodium humate could improve the surface hydrophobicity of Lactobacillus plantarum for 112.5%~128.57%. In conclusion,choosing the right concentration of sodium humate could improve the inhitition and adhesion ability of Lactobacillus plantarum.