Fengycin对苹果青霉病致病菌扩展青霉产展青霉素的抑制机制研究

付瑞敏; 张利娟; 张红; 陈五岭

doi:10.13386/j.issn1002-0306.2019.22.018

Fengycin对苹果青霉病致病菌扩展青霉产展青霉素的抑制机制研究

Mechanism of Inhibition Effect of Fengycin on Patulin Produced by Penicillium expansum

摘要

摘要: 目的:为了考察脂肽类抗生素Fengycin对扩展青霉合成真菌毒素展青霉素的抑制机制。方法:采用高效液相色谱法检测单位质量扩展青霉菌丝体产展青霉素的含量,并用SYBR GreenI Real-Time PCR方法检测Fengycin处理下扩展青霉菌丝体和未经Fengycin处理的扩展青霉菌丝体中合成展青霉素的2个关键基因6-MSAS和IDH的表达水平。结果:经过Fengycin处理,单位质量扩展青霉菌丝体产毒素含量显著下降。扩展青霉6-MSAS基因表达水平显著降低,只有对照组的11%,而IDH基因的表达水平降低差异不显著。结论:Fengycin抑制展青霉素的合成可通过抑制6-MSAS基因的表达来完成。

Abstract: Objective:In order to investigate the mechanism of inhibition effect of a lipopetide Fengcin on patulin production of Penicillum expansum. Methods:HPLC was used to detect patulin content in every unit weight of Penicillum expansum mycelium. The expression level of two key genes(6-MSAS and IDH),which translated patulin in Penicillium expansum mycelium,were detect by SYBR GreenI real-time PCR under Fengycin-treated and untreated conditions. Results:With Fengycin treatments,not only the patulin content in every unit weight of Penicillum expansum mycelium but also the expression level of 6-MSAS decreased significantly. The expression level of 6-MSAS of Fengycin-treated group was 11% folds of control group. However,the expression level of IDH of Fengycin-treated group decreased slightly,which was not significant. Conclusion:Fengycin could inhibit Penicillium expansum from producing patulin by affecting the transcriptional level of 6-MSAS.