利用LPS诱导胚胎期斑马鱼炎症模型研究羊栖菜多酚抗炎机制

倪立颖; 邹娅雪; 付晓婷; 段德麟; 许加超; 高昕

doi:10.13386/j.issn1002-0306.2019.21.046

利用LPS诱导胚胎期斑马鱼炎症模型研究羊栖菜多酚抗炎机制

Anti-inflammatory Mechanism of Phenolic Compounds from Sargassum fusiforme by LPS-induced Zebrafish Embryo Model

摘要

摘要: 为研究羊栖菜多酚对脂多糖(Lipopolysaccharides,LPS)诱导的斑马鱼的抗炎作用及机制,本文优化了LPS诱导斑马鱼炎症模型,并以卵黄囊大小、心跳速率、NO生成率、细胞死亡率和活性氧(ROS)生成率等指标比较了不同浓度羊栖菜多酚的抗炎活性。结果表明,羊栖菜多酚(50、100、200 μg/mL)对最优LPS诱导浓度(8 μg/mL)引起的炎症斑马鱼有保护作用,且呈剂量依赖性。多酚浓度为200 μg/mL时表现最优,显著降低心跳速率(102.56%,P<0.05)和卵黄囊大小(109.18%,P<0.05),NO生成率、细胞死亡率和ROS生成率分别由202.48%、168.45%和353.44%降至75.62%、101.25%和128.91%。本研究首次通过斑马鱼模型揭示了羊栖菜多酚的体内抗炎作用,为其在保健食品和化妆品中的应用提供了理论基础。

Abstract: The anti-inflammatory activities and mechanisms of phenolic compounds from Sargassum fusiforme(SFPC)were studied in lipopolysaccharides(LPS)-induced zebrafish model. The optimal LPS-induced inflammatory model in zebrafish was established,and the anti-inflammatory activities of SFPC with different concentrations were investigated by determination of yolk sac size,heart-beating rate,NO production rate,cell death rate and reactive oxygen(ROS)production rate. The results showed that SFPC(50,100,200 μg/mL)had a protective effect on inflammation induced by LPS(8 μg/mL)in a dose-dependent manner. The optimal concentration of 200 μg/mL SFPC significantly reduced the heart-beating rate(102.56%,P<0.05)and yolk sac size(109.18%,P<0.05). The rates of NO production,cell death and ROS production decreased from 202.48%,168.45% and 353.44% to 75.62%,101.25% and 128.91%,respectively. In this study,the zebrafish model was used for the first time to reveal the anti-inflammatory activity of SFPC in vivo. This study provides a theoretical basis for the application of SFPC as anti-inflammatory agent in functional food and cosmetics.