Abstract: In this study,eight kinds of biogenic amines(tryptamine,β-phenylethylamine,putrescine,cadaverine,histamine,tyramine,spermidine,and spermine)in Pixian broad bean paste with different fermentation time(1~96 months)were analyzed by HPLC method. The result showed that eight biogenic amine standards showed good linearity within 1~60 mg/L. The average recoveries,limits of detection and limits of quantitation were 73.10%~104.13%,0.17~0.35 mg/L and 0.55~0.92 mg/L,respectively,which demonstrated good precision and sensitivity of this method. Putrescine,cadaverine,tyramine and histamine were the predominant biogenic amines in broad bean pastes. With the increase of fermentation time,the content of putrescine increased rapidly,the content of cadaverine increased rapidly in 1~54 months during fermentation,then decreased significantly,and the content of tyramine and histamine increased after the 54th months of fermentation. The contents of other biogenic amines were relatively low and remained stable among the period. The total biogenic amine contents increased from(37.86±6.69) mg/kg to(155.09±8.53) mg/kg,its content increased rapidly in the 1~54 months during fermentation and then stabilized.Although there were no current regulations that restricted the contents of biogenic amines in food,the Pixian broad bean pastes used in present study were relatively safe according to the results of previous toxicological studies.