Abstract: The Acanthopanax senticosus extracts were obtained by the complex enzyme assisted ultrasonic method and the response surface methodology was used to optimize the extraction process. The components were analyzed by LC/MS,and the antioxidant activity of the extracts was evaluated by measuring the scavenging ability of DPPH free radicals and ABTS⁺ radicals and determining the total antioxidant capacity. The results showed that the optimal technological conditions were as follows:The amount of complex enzyme was 1.3%,the ratio of pectinase was 0.60,the temperature of enzymolysis was 60℃,pH was 4.2,the time of enzymolysis was 90 min,the concentration of ethanol was 60% with a volume of 150 mL,the ultrasonic power was 200 W,the ultrasonic time was 40 min and the ultrasonic temperature was 40℃. At these conditions,the extraction amount of the total glycosides from Acanthopanax senticosus was(3.33±0.05) mg/g,which was 65.69% higher than that of the conventional reflow methodwhich was(2.01±0.09) mg/g. The extracts were suspected to contain six phenols,three fatty acids,three glycosides,one saccharide derivative,one coumarin,and one terpenoids. Meanwhile,the extracts showed good scavenging ability of DPPH free radicals and ABTS⁺,and the clearance rates of DPPH free radicals and ABTS⁺ for extracts at a concentration of 1.2 mg/mL were 95.41% and 84.71%,respectively. In addition,the extracts also showed certain total antioxidant capacity in proportion to the extracts concentration. The results provide scientific information and theoretical basis for the application of Acanthopanax senticosus and its extracts in the development of antioxidant foods.