50 Hz低频电磁场对酿酒酵母蛋白组及相关代谢通路的影响

蔡杏华; 任浩锋; 杨慧; 林日辉; 蒋满州

doi:10.13386/j.issn1002-0306.2019.18.012

50 Hz低频电磁场对酿酒酵母蛋白组及相关代谢通路的影响

Effects of 50 Hz Low Frequency Electromagnetic Fields on the Proteome and Related Metabolic Pathways of Saccharomyces cerevisiae

摘要

摘要: 为了从蛋白和代谢水平进一步探究LF-EMF的分子机制,本文采用SWATH-MASS技术测定50 Hz LF-EMF辐射处理后的酿酒酵母蛋白组变化,得到的差异蛋白数据经过Unitprot数据库匹配,并对显著差异表达蛋白进行相关代谢通路分析。结果表明,50 Hz LF-EMF可显著影响酿酒酵母胞内12种蛋白的表达水平(P<0.05),包括3种上调蛋白(↑15.78%~34.21%)和9种下调蛋白(↓14.43%~40.59%)。上调的蛋白主要富集在自由基代谢、碳代谢、氨基酸生物合成和甘油脂代谢等代谢通路上;下调的蛋白主要富集在肌醇磷酸代谢、氮代谢、谷胱甘肽代谢、嘧啶代谢、嘌呤代谢和抗生素的生物合成等通路上;此外,下调表达的肌醇三磷酸合酶同时会导致其相关代谢物三磷酸肌醇的代谢水平下降(P<0.05)。因此,LF-EMF辐射可影响酿酒酵母蛋白的表达水平及其相关产物的代谢水平。

Abstract: To further explore the molecular mechanism of LF-EMF at protein and metabolism levels,the proteomic analysis of Saccharomyces cerevisiae irradiated by 50 Hz LF-EMF were determined by SWATH-MASS. The differentially expressed proteins were matched with Unitprot database,and the metabolic pathways of differentially expressed proteins were analyzed. The results showed that 50 Hz LF-EMF could significantly affect the expression level of 12 kinds of proteins in Saccharomyces cerevisiae(P<0.05),including 3 up-regulated proteins(↑15.78%~34.21%)and 9 down-regulated proteins(↓14.43%~40.59%). The up-regulated proteins were mainly concentrated in the pathway of free radical metabolism,carbon metabolism,amino acid biosynthesis and glycerolipid metabolism,while the down-regulated proteins were mainly enriched in the pathway of inositol phosphate metabolism,nitrogen metabolism,glutathione metabolism,pyrimidine metabolism,purine metabolism and antibiotic biosynthesis. In addition,the differentially expressed proteins(such as inositol triphosphate synthase)would also affect the production of its related metabolites(such as inositol triphosphate)significantly(P<0.05). Therefore,LF-EMF radiation could affect the expression level of proteins and the metabolic level of its related products in S. cerevisiae.