葛仙米藻蓝蛋白和藻红蛋白的体外抗炎和抗紫外活性比较研究

余佳; 张瑞华; 王生; 许文琦; 王玉兰

doi:10.13386/j.issn1002-0306.2019.17.049

葛仙米藻蓝蛋白和藻红蛋白的体外抗炎和抗紫外活性比较研究

Comparative Studies on the Anti-Inflammatory and Uvioresistant Activity of Phycocyanin and Phycoerythrin in Nostoc sphaeroides Kutzing in Vitro

摘要

摘要: 目的:对葛仙米藻蓝蛋白和藻红蛋白的抗炎和抗紫外活性进行体外比较研究。方法:通过Lipopolysaccharide(LPS)诱导RAW264.7细胞分泌NO和TNF-α,比较葛仙米中藻蓝蛋白和藻红蛋白的抗炎活性;通过Ultraviolet radiation B(UVB)辐照诱导NIH-3T3成纤维细胞损伤,考察藻蓝蛋白和藻红蛋白的抗紫外损伤活性。结果:体外抗炎结果显示LPS能极显著诱导RAW264.7细胞分泌NO和TNF-α(P<0.01),藻蓝蛋白和藻红蛋白均能极显著抑制LPS诱导RAW264.7分泌的NO和TNF-α的含量水平(P<0.01),其中藻红蛋白抑制NO分泌能力高于藻蓝蛋白,藻蓝蛋白对TNF-α的分泌抑制作用高于藻红蛋白。体外抗紫外损伤结果显示UVB 4000 mJ/cm²可以成功诱导NIH-3T3细胞的紫外损伤,但是藻蓝蛋白和藻红蛋白对NIH-3T3细胞的紫外损伤并无明显保护作用。结论:葛仙米中藻蓝蛋白和藻红蛋白具有很好的体外抗炎活性,但两者对UVB诱导的细胞损伤并无明显保护作用。

Abstract: Objective:Comparative studies on the anti-inflammatory and uvioresistant activity of phycocyanin and phycoerythrin in Nostoc sphaeroides Kutzing were developed in vitro. Methods:Raw264.7 cells induced by LPS were used to evaluate the anti-inflammatory activity through detecting NO and TNF-α secretion. NIH-3T3 cell damaged by UVB was used to evaluate the uvioresistant activity. Results:Raw264.7 cells induced by LPS secreted NO and TNF-α extremely significantly(P<0.01),and phycocyanin and phycoerythrin extremely significantly reduced the level of NO and TNF-α secretion(P<0.01). Phycoerythrin was more effective than phycocyanin on the ability of NO secretion inhibition. And phycocyanin was more effective than phycoerythrin on the ability of TNF-α secretion inhibition.UVB 4000 mJ/cm²inhibited the viability of NIH-3T3 cells successfully,but phycocyanin and phycoerythrin showed no protection efficacy on UVB induced cells damage.Conclusion:Phycocyanin and phycoerythrin shows significant anti-inflammatory activity,but there is no protection efficacy on UVB induced cells damage.