Abstract: To investigate the effects of ozone treatment on the storage quality of postharvest Flammulina velutipes,five different concentrations of ozone were used before the storage under 4℃. Several parameters such as sensory evaluation,weight loss rate,total soluble solids,soluble protein,malonaldehyde(MDA),peroxidase(POD),and polyphenol oxidase(PPO)activity,were determined to evaluate the effect of ozone treatment. The results indicated that ozone treatment maintained good sensory quality and protein content,reduced the decline of the content of total soluble solids,inhibited the increase of MDA and PPO activities,and delayed the changes of PPO activity. Among these five ozone concentrations,the highest efficiency was observed by using 16.6 mg/L for flammulina velutipes treatment. This study suggests that ozone treatment might be a promising candidate for maintaining flammulina velutipes quality and extending its postharvest life.