热变性牛血清白蛋白对三种活性物质的复合和保护作用

董焕焕; 乌斯嘎勒; 冯稳稳; 梁丽

doi:10.13386/j.issn1002-0306.2019.15.010

热变性牛血清白蛋白对三种活性物质的复合和保护作用

Effect of Heat-denatured Bovine Serum Albumin on Complexation and Protection of α-tocopherol,Resveratrol and(-)-epigallocatechin-3-gallate

摘要

摘要: 本文通过竞争实验证明热变性牛血清白蛋白(hBSA)同时结合α-生育酚、白藜芦醇和表没食子儿茶素没食子酸酯(EGCG)的可能性,通过结构稳定性和抗氧化稳定性实验表征蛋白对活性物质的保护情况。结果表明hBSA能同时结合三种物质,并且在复合物的形成过程中不存在竞争行为。储藏48 h后,游离态α-生育酚、白藜芦醇、EGCG残留率分别为48%、68%、0%,hBSA-EGCG-α-生育酚-白藜芦醇复合物中残留率分别为91%、90%、15%;储藏288 h后,游离态的α-生育酚、白藜芦醇、EGCG对ABTS自由基的清除能力分别减少了6%、10%、31%,hBSA-EGCG-α-生育酚-白藜芦醇复合物仅减少了13%。因此,hBSA是同时负载三种活性物质的良好载体。研究结果将为蛋白类共包埋载体的开发提供更多参考依据。

Abstract: In this paper,the possibility of heat-denatured BSA (hBSA) binding simultaneously to α-tocopherol,resveratrol and epigallocatechin gallate (EGCG) was demonstrated by competitive experiments,and the protection of hBSA on bioactive components was characterized by structural and antioxidant stability experiments.The results indicated that hBSA bind the three bioactive simultaneously whereas no competition was found between these components. The content of free α-tocopherol,resveratrol and EGCG decreased to 48%,68% and 0% respectively after 48 h storage. On the other hand,the content of α-tocopherol,resveratrol and EGCG after 48 h were 91%,90% and 15% for hBSA-EGCG-α-tocopherol-resveratrol complexes. The reduction in ABTS scavenging capacity of free α-tocopherol,resveratrol and EGCG were 6%,10% and 31% respectively after 288 h,however,13% in the ABTS scavenging capacity of hBSA-EGCG-α-tocopherol-resveratrol complexes was observed. In conclusion,hBSA is a good carrier for loading three active substances simultaneously. The data gathered here can be useful for the development of protein-based encapsulating carriers.