GP-MSE/GC-MS分析夏枯草不同部位挥发性成分

杨翠; 赵锦花

doi:10.13386/j.issn1002-0306.2019.13.029

GP-MSE/GC-MS分析夏枯草不同部位挥发性成分

杨翠,
赵锦花

Analysis of Volatile Components from Different Parts of Prunella vulgaris by GP-MSE/GC-MS

摘要

摘要: 采用气流吹扫微萃取法(GP-MSE)提取夏枯草中挥发性成分,以GC-MS进行成分分离与鉴定,以峰面积归一化法进行定量,运用化学和PCA法比较其根、枝、叶、花中挥发性成分的种类和含量差异。结果表明:共分离出145种挥发性成分,根、枝、叶、花分别定性98种、98种、98种、124种成分,分别占各部位挥发性成分总量的66.53%、91.24%、89.32%和84.87%。花中主要含有烷烃类(29.01%)和含N、O-类物质(12.17%)。根和枝中主要贡献成分均为醛类(20.66%、21.18%)、酚类(13.23%、19.80%),叶中主要贡献成分为醇类(26.79%)和醛(15.15%)。结果显示花中成分与其他部位存在较大差异,而其他各部位间差异不大。本研究为夏枯草在食品、药品中进一步开发利用提供参考依据。

Abstract: The volatile components in Prunella vulgaris were extracted by gas purge micro-extraction technique, and then analyzed by gas chromatography-mass spectrometry, the relative contents of individual constituents in the volatile components were determined by peak area normalizing method, the volatile components of root, branch, leaf and flower for Prunella vulgaris were compared by chemical method and principal component analysis. The results showed 145 peaks were isolated in Prunella vulgaris, 98, 98, 98 and 124 components were identified in root, branch, leaf and flower, which accounted for 66.53%, 91.24%, 89.32% and 84.87% of the total peaks in each part. Flower mainly contains alkanes (29.01%) and N, O-compounds (12.17%). Aldehydes (20.66%、21.18%) and phenols (13.23%、19.8%%) were the main contributing components in root and branch. Alcohols (26.79%) and aldehydes (15.15%) were the main contributing components in leaf. The results showed that the chemical components were a great difference between the flower and the other parts. This study provides reference basis for development and utilization of Prunella vulgaris in food and medicine.