Abstract: The auto-aggregation and hydrophobicity of six human origin probiotics were determined,and their adhesion ability in vitro cell adhesion models was evaluated using the human colon cancer cell lines Caco-2 and HT-29. Finally,the effects of growth stage,cell concentration and incubation time on the adhesion of Lactobacillus fermentum CECT5716 were investigated. The results showed that the auto-aggregation rate of Lactobacillus acidophilus F-1 was the highest(69.92%). And the most hydrophobic was Lactobacillus rhamnosus MP-108(90.10%). Bifidobacterium lactis AD011 had the strongest adhesion to Caco-2 and HT-29,(42.80±0.68),(47.01±0.20) CFU/cell,respectively. There were strain specificity in the auto-aggregation ability,hydrophobicity and adhesion ability among different probiotics,but there was no significant correlation among the auto-aggregation ability,hydrophobicity and adhesion ability. In addition,the adhesion ability of L. fermentum CECT5716 improved significantly(p<0.05)with the increase of the concentration of the bacteria,but the adhesion ability no longer increased obviously when the concentration of the bacteria reached 10⁸ CFU/mL or higher than it. As the time of co-incubation prolonged,the adhesion ability was also enhanced,but it had no longer increased after 2 h of co-incubation;and the cell exhibited the strongest adhesion ability in the stationary phase,reaching(3.61±0.20)CFU/cell. Most of the probiotics examined had strong potential adhesion,of which B. lactis AD011 was the strongest. These probiotics have potential probiotic properties of colonization and growth in human intestinal tract,and have good application prospects in food and pharmaceutical industries.