洋甘菊各萃取相抗氧化活性及其有效成分分析

楚秉泉; 方若思; 李玲; 肖功年; 袁海娜; 龚金炎

doi:10.13386/j.issn1002-0306.2019.08.001

洋甘菊各萃取相抗氧化活性及其有效成分分析

Antioxidant Activities and Analsis of Active Components in Different Extract Phases of Chamomile

摘要

摘要: 目的:筛选洋甘菊醇提物抗氧化活性最强的萃取组分,并对该组分抗氧化活性的有效成分进行研究。方法:采用DPPH·、ABTS⁺·、O₂^-·自由基清除能力和FRAP抗氧化功能测试方法,对比研究了洋甘菊醇提物不同极性萃取组分的抗氧化活性,并运用HPLC等方法对抗氧化能力最强萃取组分进行有效成分分析。结果:洋甘菊乙酸乙酯萃取组分的抗氧化能力最强,对DPPH·、ABTS⁺·和O₂^-·的IC₅₀分别为25.6、30.6和83.3 mg/mL,总抗氧化能力(以trolox计)为16.7 mg/mL。成分分析表明,乙酸乙酯萃取相中总酚和总黄酮含量分别为25.84%和14.93%,在检测到的10种多酚或黄酮类化合物中,木犀草素、蒙花苷和木犀草素-7-葡萄糖含量较丰富。结论:洋甘菊醇提物的乙酸乙酯萃取组分具有良好的抗氧化能力可能与其富含的多酚和黄酮成分有关。

Abstract: Objective:The purpose was to obtain the extraction fraction with the strongest antioxidant activity of the chamomile alcohol extracts,and thereby to explore its active components. Methods:The methods such as DPPH·,ABTS⁺·,O₂^-· and FRAP were used to compare the antioxidant activities of different polar fractions of chamomile alcohol extracts. And then,the compositions of the strongest antioxidant fraction were analyzed by HPLC. Results:The ethyl acetate fraction of chamomile alcohol extracts had the strongest antioxidant capacity. The IC₅₀ of ethyl acetate fraction for DPPH·,ABTS⁺· and O₂^-· were 25.6,30.6 and 83.3 mg/mL respectively,and the total antioxidant capacity(presented as trolox)was 16.7 mg/mL. The composition analysis showed that the contents of total phenol and total flavonoid in the ethyl acetate fraction were 25.84% and 14.93%,respectively. And the contents of luteolin,glucoside and luteolin-7-glucose were the highest among the 10 polyphenols or flavonoids in the ethyl acetate phase. Conclusion:The good antioxidant activity of acetate extracts of the extract of chamomile alcohol might be related to its rich polyphenols and flavonoids.