Abstract: Objective:To study the effects on key enzymes glycolysis and oxidative phosphory-lation of Stichopus japonicus crude polysaccharides (SJP). Methods:SJP were extracted by enzymatic hydrolysis (papain, pepsin and trypsin), the experimental mice were divided into normal control group, SJP 1 group (papain hydrolysis method prepared by SJP), SJP 2 group (pepsin and trypsin hydrolysis method prepared by SJP), 8 mice per group. The gastric dose was 200 mg/kg/d for 10 days, administered daily at 9:00 am, and the normal control mice were given an equal volume of normal saline.The effects of SJP on the glycolytic key enzymes (hexokinase, 3-glyceraldehyde phosphate dehydrogenase, lactate dehydrogenase) and aerobic oxidation key enzymes (pyruvate dehydrog-enase, mitochondrial complexⅠ, mitochondrial complex Ⅴ) were determined. Results:Compared with the control group, the activities of HK, GAPDH and LDH in the SJP group were decreased, and there was significant difference (p<0.05), the activities of PDH, mitochondrial complex I and complex V in SJP were increased, and there was significant difference (p<0.05), that was SJP could inhibit the activities of hexokinase, 3-glyceraldehyde phosphate delydrogenase and lactate dehydrogenase, increased the activities of pyruvate dehydogenase, mitochondrial complex Ⅰ and mitochondrial complex Ⅴ. Conclusion:SJP could inhibit glycolysis and promote the aerobic oxidation. The main characteristic of cancer cells is that the energy is produced through glycolysis, and it is inferred that SJP has a role in preventing cancer.