基于肽生物标志物技术鉴别肉品的真实性研究进展
Research Progress in Identification of Meat Authenticity based on Peptide Biomarker Technology
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摘要: 基于DNA和蛋白质的方法已广泛应用于肉制品的真实性鉴定,但肉制品经加工处理后,DNA和蛋白质很容易发生降解和变性,而蛋白质的一级结构(氨基酸序列)则足够稳定。伴随着蛋白质组学的发展,以肽生物标志物为基础的鉴定方法逐渐应用于肉制品的真实性分析。本文将从肉中肽生物标志物的分离鉴定方法、肽生物标志物在鉴定不同肉制品及肉制品中外源蛋白的应用三个方面进行归纳和总结,并对肽生物标志物在鉴定肉品真实性应用中的发展方向进行展望,以期为食品溯源提供更丰富的技术手段。Abstract: The methods authenticity identification of meat products based on DNA and protein have been widely used in food research, but DNA and protein are prone to denaturation and degradation in highly processed meat samples, however primary structure of the protein (amino acid sequence) is usually stable. With the development of proteomics, the identification methods based on peptide biomarkers have gradually been applied to the authenticity analysis of meat products. In this paper, the analysis and identification methods of peptide biomarkers in meat, the application of peptide biomarkers in identifying different meat products and non-meat origin in meat products were reviewed. The development trend of peptide biomarkers in the identification of meat authenticity was used to provide a richer technical means for food traceability.