Abstract: Macroporous adsorption resins were utilized for purification of triterpenes from the crude extracts of Trametes orientalis. Hepatoprotective effect of triterpenes from Trametes orientalis was evaluated by an alcohol-induced liver injury model in mice. The results revealed that X-5 resin offered the best adsorption and desorption properties for triterpenes from Trametes orientalis. The adsorption equilibrium datas of triterpenes on X-5 resin were fitted to the Langmuir model. Optimal purification parameters were as follows:Loading concentration, loading flow rate, ethanol concentration, and elution flow rate were 2.34 g/L, 2 BV/h, 70% and 2 BV/h, respectively. The purity of triterpenoids purified by X-5 resin was 83.30%, which was 3 times higher than crude extracts. X-5 resin had excellent performance and could be applied to purify triterpenes from Trametes orientalis. The increased serum alanine aminotransferase and aspartate aminotransferase levels of mice after alcohol treatment were inhibited by the triterpenes administration extremely significant (p<0.01). Furthermore, the triterpenes could effectively increase the superoxidase dismutase, catalase and glutathione peroxidase activities, and inhibit the increase in malondialdehyde level extremely significant (p<0.01). These results indicated that the triterpenes from Trametes orientalis might be of therapeutic value in ameliorating the hepatotoxicity caused by alcohol.