甘草甜味素的化学成分研究

张航航; 葛振红; 李国柱; 贾旭; 孟庆艳; 刘文杰

doi:10.13386/j.issn1002-0306.2019.02.039

甘草甜味素的化学成分研究

Study on Chemical Constituents of Licorice Sweeteners

摘要

摘要: 目的:研究提取甘草中甘草酸后剩余的副产品甘草甜味素中的化学成分,旨在探明甘草甜味素中的化学成分,拓宽其应用范围,为评价化学成分的生物活性提供科学依据。方法:采用溶剂提取、中压制备色谱和循环制备液相色谱等手段对甘草甜味素中的化学成分进行分离纯化,通过核磁共振对化合物结构进行鉴定。结果:从甘草甜味素中分离鉴定出10个化合物,分别为对羟基苯甲酸(p-hydroxybenic acid,I)、甘草素-7,4'-二葡萄糖苷(Liquiritigenin-7,4-diglucoside,II)、甘草素-4'-O-芹糖基-(1→2)葡萄糖苷(Liquiritigenin-apiosyl(1→2)glucoside),III)、异甘草素葡萄糖芹菜苷(Licuraside,IV)、异甘草苷(Isoliquiritin,V)、甘草素(Liquiritigenin,VI)、异甘草素(Isoliquiritigenin,VII)、芒柄花素(Formononetin,VIII)、甘草苷(Liquiritin,IX)、刺甘草查尔酮(Echinatin,X)。结论:该方法能分离得到10种成分,能拓宽副产品的应用范围。

Abstract: Objective:The chemical constituents of licorice sweeteners which were the by-products after glycyrrhizic acid extracted were stuided to ascertain the chemical constituents of licorice sweeteners, widen its application scope and provide a scientific basis for evaluating the biological activity of the chemical components. Methods:The chemical constituents of licorice sweeteners were separated and purified by solvent extraction, medium pressure preparative chromatography and recycling preparation liquid chromatography. Meanwhile, NMR methods were employed to identify their structures. Results:Ten compounds were isolated and identified as p-hydroxybenic acid, Liquiritigenin-7, 4'-diglucoside, Liquiritigenin-apiosyl (1→2) glucoside, Licuraside, Isoliquiritin, Liquiritigenin, Isoliquiritigenin, Formononetin, Liquiritin, Echinatin.Conclusion, The method can separate 10 components and widen the application range of by-products.