Abstract: In this paper, the crude polysaccharides from germinated brown rice were purified. The decolorization effects of activated carbon adsorption, hydrogen peroxide oxidation and macroporous resin adsorption were compared. The Sevage method, trichloroacetic acid method, enzyme and Sevage method were compared. These method was to screen out the best method for decolorization and deproteinization of germinated brown rice polysaccharide. The chromatographic purification effects of DEAE-Sepharose CL-6B, DEAE Fast Flow and DEAE Sepharose 52 on crude polysaccharides from brown rice were compared and the optimal fillers were screened. The molecular weight of each component of the purified germinated brown rice polysaccharide was measured. The results showed that macroporous resin AB-8 had the best decolorization effect on germinated brown rice polysaccharide, the decolorization rate was 86.57%, and the polysaccharide loss rate was 28.96%. The enzyme-Sevage method had good deproteinization effect and low polysaccharide loss rate, the deproteinization rate was 74.36%, and the polysaccharide loss rate was 14.09%. The best filler for column chromatography of germinated brown rice polysaccharide was DEAE-Sepharose CL-6B. The weight average molecular weight was calculated according to the linear regression equation. The average molecular weight of the washed polysaccharide component was 1.47×10⁵ Da, and the average molecular weight of the salt washed polysaccharide component was 9.62×10⁵, 5.59×10⁶, 3.15×10⁵ Da, respectively. Conclusion:The method for removing protein and decolorization of crude polysaccharide from germinated brown rice was determined, and the best column chromatography packing was selected. Four components of germinated brown rice polysaccharide were isolated to extract, purify and separate the crude polysaccharide from germinated brown rice, which provided a theoretical basis for the transformation of polysaccharides into industrial production.