普洱茶对大鼠肥胖的干预和保肝护肝作用
Effects of Pu-erh Tea on the Intervention of Obesity and Protection of Liver in Rats
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摘要: 将SD大鼠随机分为正常对照组、高脂对照组、普洱茶低(0.45 g/kg)、中(0.90 g/kg)、高(1.35 g/kg)浓度组,每组10只,每天灌胃普洱茶,连续处理十二周后,禁食24 h,取大鼠肝脏,测其甘油三酯(TG)、总胆固醇(TC)的含量、腺苷酸活化蛋白激酶(AMPK)的表达及与脂代谢相关的基因表达,以研究普洱茶的预防肥胖和保肝护肝作用。结果显示,普洱茶能降低高脂饲料喂养的大鼠肝脏中TG和TC的含量,显著提高腺苷酸活化蛋白激酶(AMPK)磷酸化水平(p<0.05),极显著下调脂肪酸合成酶(FAS)、固醇调节元件结合蛋白1c(SREBP-1c)和羟甲基戊二酰辅酶A还原酶(HMGCR)的mRNA表达水平(p<0.01),作用效果呈剂量依赖性。因此,普洱茶具有良好的预防肥胖和保肝作用效果。Abstract: Sprague dawley (SD) rats were randomly divided into normal-diet control group,high-fat diet control group,and three levels groups (each group with 10 rats) treated with high (1.35 g/kg),medium (0.90 g/kg) and low (0.45 g/kg) hot water extracts from Pu-erh tea. The rats were lavaged every day for continuous administration for 12 weeks. After fasted for 24 h,the rats were sacrificed and the livers were used as materials. The contents of triglyceride (TG) and cholesterol (TC),the expression levels of phosphorylated adenosine monophosphate (AMP) -activated protein kinase (p-AMPK) protein and the mRNA related to lipid metabolism were determined for study Pu-erh tea's roles on intervention of obesity and protection of liver. The results showed that Pu-erh tea could reduce the contents of TG and TC,improving the level of AMPK phosphorylation (p<0.05),up-regulating the mRNA levels of FAS,SREBP-1c and HMGCR (p<0.01). The effects were in dose-dependent characteristics. Therefore,Pu-erh tea had good functions of preventing obesity and protecting liver.
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