超声波辅助酶解提取鱼皮胶原蛋白的工艺优化

王锡念; 徐志善; 孙钦军; 包建强

doi:10.13386/j.issn1002-0306.2019.01.032

超声波辅助酶解提取鱼皮胶原蛋白的工艺优化

Optimization of Ultrasonic Assisted Enzymatic Extraction of Collagen from Fish Skin of Lophiiformes

摘要

摘要: 以鮟鱇鱼皮为原料,以料液比、超声时间、加酶量、酶解时间、酶解温度、提取pH为实验因素,用超声-风味酶法和超声-碱性酶法分别提取,并利用正交试验确定鮟鱇鱼皮胶原蛋白最佳提取工艺。结果表明,超声-风味酶法提取鮟鱇鱼皮胶原蛋白的最佳提取工艺条件为:超声时间80 min,酶解时间5 h,风味蛋白酶5000 U/g,酶解温度40 ℃,在此条件下胶原蛋白提取率为3.54%±0.21%;超声-碱性酶法提取鮟鱇鱼皮胶原蛋白的最佳提取工艺条件为:超声时间80 min,酶解时间6 h,碱性蛋白酶4000 U/g,酶解温度45 ℃,在此条件下胶原蛋白提取率为3.25%±0.68%,其中酶解时间比超声-风味酶法多1 h,但胶原蛋白提取率却不及超声-风味酶法。综上,选取超声-风味酶法提取鮟鱇鱼皮胶原蛋白。

Abstract: The optimum extraction process of collagen from fish skin of Lophiiformes was determined by the ultrasonic flavor enzyme method and the ultrasonic alkaline enzyme method,with the ratio of material to liquid,ultrasonic time,enzyme dosage,enzyme hydrolysis time,enzyme hydrolysis time,enzyme hydrolysis temperature and pH as the experimental factors. The results showed that,the optimum extraction conditions were as follows:Ultrasonic time 80 min,enzyme hydrolysis time 5 h,flavor protease 5000 U/g,enzymolysis temperature 40℃,collagen extraction rate was 3.54%±0.21% under this condition. The optimal extraction conditions for the extraction of collagen from fish skin of Lophiiformes by ultrasonic-alkaline enzyme method were as follows:Ultrasonic time 80 min,enzyme hydrolysis time 6 h,alkaline protease 4000 U/g,enzyme hydrolysis temperature 45℃,collagen extraction rate was 3.25%+0.68%,the enzymatic hydrolysis time was 1 h higher than that of ultrasonic flavor enzyme method,but the extraction rate of collagen was less than ultrasonic flavor enzyme method. In summary,the ultrasonic flavor enzyme method was used to extract collagen from fish skin of Lophiiformes.