添加氧载体对<i>γ</i>-多聚谷氨酸发酵的影响

添加氧载体对γ-多聚谷氨酸发酵的影响

摘要: 针对γ-多聚谷氨酸(Poly-γ-glutamic acid,γ-PGA)好氧发酵过程中存在的供氧不足而导致产量偏低的问题,探究了不同氧载体对γ-PGA发酵的影响,并对最适氧载体的添加量和添加时间进行了优化。结果表明,正十六烷为最适氧载体,其最佳添加条件为:添加量为0.5%(体积比),添加时间为0 h,发酵48 h,γ-PGA产量达到最高值为(18.62±0.84) mg/mL,相比对照组提高了30%以上。本研究为通过添加氧载体,增加溶氧来提高γ-PGA发酵产量提供了理论依据。

Abstract: In order to solve the problem of low yield caused by insufficient oxygen supply during the aerobic fermentation of poly-γ-glutamic acid (γ-PGA). The effects of different oxygen-vectors on γ-PGA fermentation was studied. The addition amount and time of the most effective oxygen-vector were optimized as well. The results showed that n-hexadecane was the most effective oxygen-vector. The optimal addition condition was 0.5% (V:V) and the adding time was 0 h. After 48 h fermentation,the yield of γ-PGA was increased to (18.62±0.84) mg/mL,which increased more than 30% compared with the control group. This study provided a theoretical basis for increasing the yield of γ-PGA by addition of oxygen-vectors to increase dissolved oxygen.