Abstract: Due to its unique chemical structure,polyphenols have many physiological effects such as anti-oxidation,anti-tumor,antibacterial and so on,and are widely used in the field of food and medicine. Polyphenols and proteins combine to form complexes,which change the nutritional characteristics and functional structures of the two. A variety of experimental methods and techniques can be used to determine the binding constants,binding sites,types of interaction force,binding-site number,and polyphenol binding to proteins changes in protein conformation and physiological function as well as the influence of external conditions on polyphenol-protein binding. This review summarized the progress of research on the interaction between polyphenols and proteins,including molecular docking,UV-Vis absorption spectroscopy,fluorescence spectroscopy,circular dichroism,fourier transform infrared spectroscopy,isothermal titration calorimetry,raman spectroscopy and nuclear magnetic resonance spectroscopy. The review found that each method has some advantages and limitations. Using a variety of methods combined with analysis can more fully understand the interaction between polyphenols and proteins.