基于荧光微球的免疫层析法快速检测黄曲霉毒素B<sub>1</sub>

基于荧光微球的免疫层析法快速检测黄曲霉毒素B₁

摘要: 本研究采用荧光微球纳米粒子作为免疫标记物,偶联黄曲霉毒素B₁单克隆抗体制备纳米探针,建立了决明子中黄曲霉毒素B₁的荧光微球免疫层析定量检测方法。结果表明:方法的线性范围为0.1~2.0 ng/mL,灵敏度为0.034 ng/mL。该方法具有快速和简便等优点,可满足现场快速定量筛查的需求。

Abstract: In this study,fluorescent microsphere nanoparticles were used as immune label to develop fluorescent microspheres immunochromatographic quantitative assay for detection of aflatoxin B₁ in Semen cassiae. The results showed that the linear range and the sensitivity of the fluorescent microspheres immunochromatographic quantitative assay was 0.1~2.0 and 0.034 ng/mL,respectively. The detection methods had the advantages of rapidity and simplicity,could meet the rapid and quantitative need of the on-site screening process.