哈密瓜(<i>Cucumis melo</i> L.)致腐真菌的鉴定

乔言平; 吴朝霞; 丁真真; 王宇滨; 马越; 赵晓燕; 张超

doi:10.13386/j.issn1002-0306.2018.23.022

哈密瓜(Cucumis melo L.)致腐真菌的鉴定

Identification of Spoilage Fungi of Cantaloupe(Cucumis melo L.)

摘要

摘要: 研究从哈密瓜表面分离出引起其腐败的菌株A和菌株B,并采用显微镜形态学观察、表型特征和分子生物学三种方法对其进行鉴定。结果发现菌株A可利用碳源包括杏苷、糊精、D-半乳糖、D-葡萄糖酸、麦芽糖、肝糖、L-鼠李糖、L-山梨糖、L-乳酸等72种碳源,其ITS rDNA序列与尖孢镰刀菌甜瓜转化型(Fusarium oxysporum f.sp.melonis)的同源性为99%,分支长度为0.00。菌株B可利用碳源包括L-阿拉伯糖、D-果糖、丙三醇、蔗糖和木糖醇等54种碳源,其ITS rDNA序列与链格孢菌(Alternaria alternata)的同源性为100%,分支长度为0.00。因此,推断菌株A为尖孢镰刀菌甜瓜转化型,菌株B为链格孢菌。

Abstract: Strain A and B were isolated from the surface of the cantaloupe(Cucumis melo L.),which was further identified by the microscopic morphological observation,phenotypic characteristics and molecular methods. Strain A used the 72 kinds of carbon sources,including dextrin,D-galactose,D-gluconic acid,maltose,glycogen,L-rhamnose,L-sorbose,L-lactic acid and etc.. ITS rDNA sequence of strain A showed 99% homology with Fusarium oxysporum f. sp. melonis with the branch length of 0.00.Strain B used 54 kinds of carbon sources,including L-arabinose,D-fructose,glycerol,sucrose,xylitol and etc.. ITS rDNA sequence strain B showed 100% homology with Alternaria alternata with the branch length of 0.00. Therefore,strain A and B was supposed to be Fusarium oxysporum f. sp. melonis and Alternaria alternata,respectively.