地参结合酚提取物对α-葡萄糖苷酶和胰脂肪酶的抑制作用

杨文艺; 高春燕

doi:10.13386/j.issn1002-0306.2018.23.020

地参结合酚提取物对α-葡萄糖苷酶和胰脂肪酶的抑制作用

Inhibitory Effects of Bound Phenolic Extracts from Lycopus lucidus Turcz. on α-glucosidase and Pancreatic Lipase

摘要

摘要: 目的:探讨地参结合酚提取物对α-葡萄糖苷酶和胰脂肪酶活性的抑制作用。方法:采用X-5大孔树脂纯化地参结合酚提取物,并用Folin-酚法测定地参结合酚提取物酚含量;同时,采用HPLC对地参结合酚提取物的组成进行分析;以抑制率为考察指标,测定地参结合酚提取物对α-葡萄糖苷酶和胰脂肪酶活性的抑制作用。结果:地参结合酚提取物酚含量为155.55~164.95 μg GAE/mg,主要的酚类化合物单体为咖啡酸(40.67~72.18 μg/mg EDW)和迷迭香酸(22.01~46.22 μg/mg EDW);抑制α-葡萄糖苷酶和胰脂肪酶的IC₅₀值范围分别为2.0275~3.7538和1.4438~3.1030 mg/mL。结论:地参结合酚提取物对α-葡萄糖苷酶和胰脂肪酶抑制剂具有显著的抑制作用,可作为α-葡萄糖苷酶和胰脂肪酶抑制剂。

Abstract: Objective:To investigate the inhibitory effects of bound phenolic extracts from Lycopus lucidus Turcz.(L. lucidus)on α-glycosidase and pancreatic lipase. Methods:The phenolic content was determined by Folin-phenol method and phenolic extracts were purified through X-5 macroporous resin. Additionaly,HPLC was used to analyze the phenolic composition. The inhibitory effect of bound phenolic extracts on α-glycosidase and pancreatic lipase were evaluated with inhibition rate as index. Results:The phenolic content of bound phenolics from L. lucidus was in the range of 155.55~164.95 μg GAE/mg. Caffeic acid and rosmarinnic acid were the major pehnolic compounds with the content ranging from 40.67 to 72.18 and 22.01 to 46.22 μg/mg EDW,respectively. The IC₅₀ values of inhibitory effects on α-glycosidase and pancreatic lipase were in the range of 2.0275~3.7538 and 1.4438~3.1030 mg/mL,respectively. Conclusion:Significant inhibitory effects of bound phenolics on α-glycosidase and pancreatic lipase were observed and L. lucidus could be used as α-glycosidase and pancreatic lipase inhibitors.