Abstract: This paper aimed to compare the differences of fish quality between the two culture modes and provide valuable conduct for improvement and promotion of the industrialized pond culture. Two-month-old fish(about 5 cm in length)were randomly divided into still water culture group and flowing water culture group,which were separately cultured in traditional ponds system and in-pond raceway system(flow rate was 3 cm/s). After cultured in different ponds 12 months later,collecting the fish axis muscles of each group. It was conventional method that used to measure fish nutrient composition and texture. Fish fatty acids and amino acids was measured by Gas Chromatography-Mass Spectrometer(GC-MS)and High Performance Liquid Chromatography(HPLC).The results showed that,compared with still water culture,the contents of fish muscle protein significantly increased(p<0.05),the contents of hydroxyproline,collogen and collagen significantly increased(p<0.01),the content of fat significantly decreased(p<0.05)in flowing water culture,and muscle texture analysis of flowing water showed that the hardness,elasticity,adhesiveness and chewiness ponds significantly increased(p<0.05).Moreover,the content of muscle polyunsaturated fatty acid(PUFA),omega-6 fatty acids,oleic acid,linoleic acid,eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)significantly increased in flowing water. The content of essential amino acids,especially glutamic acid(Glu),valine(Val),leucine(Leu),isoleucine(Ile),significantly increased(p<0.05). Therefore,compared to traditional pond culture,the bass muscle quality of industrialized pond culture was more firm and chewy.