海南4种茶水提物的抗氧化及抑菌活性比较

李花; 陈国德; 李培征; 骆焱平

doi:10.13386/j.issn1002-0306.2018.23.010

海南4种茶水提物的抗氧化及抑菌活性比较

Antioxidant and Antibacterial Activity of Aqueous Extracts from Four Kinds of Tea in Hainan

摘要

摘要: 为比较海南苦丁茶、白沙绿茶、大叶种茶及鹧鸪茶4种茶水提物的抗氧化及抑菌活性,本实验通过水浸提法获得4种茶的水提物,利用分光光度法测试其体外抗氧化活性;并以5株酿酒酵母及5种致病细菌为靶标,分别测定了4种茶水提物的抗氧化活性及抑菌活性。结果表明:苦丁茶、白沙绿茶、大叶种茶及鹧鸪茶水提物的提取率分别为29.31%、23.02%、27.07%和16.18%;总多酚含量分别为(251.48±12.30)、(315.05±10.98)、(395.05±27.70)、(420.05±25.65)mg GAE/g DW;总黄酮含量分别为(691.60±19.47)、(292.60±18.36)、(524.93±1.53)、(217.60±7.00)mg RE/g DW;4种茶的总抗氧化能力结果由强到弱为:大叶种茶>鹧鸪茶>白沙绿茶>苦丁茶;特别是鹧鸪茶和大叶种茶对二苯基苦酰肼基(Diphenyl picryl hydrazinyl radical,DPPH)自由基、2'-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)自由基及超氧阴离子的清除率均高于标准品芦丁和特丁基对苯二酚(Terbutyl hydroquinone,TBHQ)。同时4种茶对H₂O₂氧化应激胁迫下的5株酿酒酵母模式菌株的存活率均有提高,其中苦丁茶对基因缺陷型菌株gsh1Δ的存活率提高最明显,为30.21%。4种茶对致病细菌有较好的抑制作用,其中大叶种茶对金黄色葡萄球菌抑制效果最优,最小抑菌浓度(Minimum inhibitory concentration,MIC)为1.56 mg/mL。由此表明,4种茶水提物具有不同的抗氧化活性和抑菌活性,且多酚含量与活性之间具有较好的相关性。

Abstract: In order to compare the antioxidant activity and antibacterial activity of four kinds of tea extracts from Ilex kudingcha C. J. Tseng,Baisha green tea,C. sinensis var. assamica cv. Hainan-dayezhong and Mallotus oblongifolius Muell. Arg. in Hainan,water-extract of four kinds of tea was obtained by water-immersion extraction,and antioxidant activity in vitro was determinated by spectrophotometry. The antioxidant and antibacterial activities of four kinds of tea extracts were determined respectively to choose five strains of Saccharomyces cerevisiae and five kinds of pathogenic bacteria as targets. The results showed that the extraction rates of Ilex kudingcha,Baisha green tea,C.sinensis and Mallotus oblongifolius were(251.48±12.3),(315.05±10.98),(395.05±27.70),(420.05±25.65) mg GAE/g DW,respectively. The total flavonoids contents were(691.60±19.47),(292.60±18.36),(524.93±1.53),(217.60±7.00) mg RE/g DW,respectively. The total antioxidant activity of the four kinds of tea changed from strong to weak as:C. sinensis > Mallotus oblongifolius > Baisha green tea > Ilex kudingcha,especially Mallotus oblongifolius and C. sinensis on DPPH free radicals,ABTS free radicals and superoxide anion clearance were higher than that of the standard rutin and TBHQ. At the same time,the survival rate of five strains S. cerevisiae under H₂O₂ oxidative stress was increased. The survival rate of Ilex kudingcha was significantly increased to 30.21% against gsh1Δ. The four kinds of tea had a good inhibitory effect on pathogenic bacteria,of which C. sinensis best inhibitory effect on Staphylococcus aureus,its MIC value of 1.56 mg/mL. It showed that the four tea extracts had different antioxidant and bacteriostatic activities,and there was a good correlation between the polyphenol content and the activity.