藿香叶黄酮提取工艺优化及其体外抗氧化活性

艾薇; 李悦; 匡建秋

doi:10.13386/j.issn1002-0306.2018.22.033

藿香叶黄酮提取工艺优化及其体外抗氧化活性

Extraction Technology of Flavonoids from the Leaf of Agastacbe rugose (Fisch.et.Mey.)O. Ktze and Its Antioxidant Activities in Vitro

摘要

摘要: 对藿香叶黄酮提取工艺及体外抗氧化活性进行研究。应用微波辅助乙醇提取法提取保山藿香叶中的黄酮,并通过单因素实验和L₉(3⁴)正交设计对提取工艺进行优化,并以抗坏血酸为对照,对其还原力及羟自由基(·OH)、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)及2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS⁺·)活性的清除能力进行探讨。结果表明:藿香叶黄酮提取的最佳条件为料液比1:60 g/mL,乙醇浓度60%,微波功率567 W,提取时间70 s,提取得率可达6.11%。此条件下得到的藿香叶黄酮提取物清除几种自由基的能力良好,尤其对ABTS⁺·清除能力最优,对·OH、DPPH·和ABTS⁺·IC₅₀分别为281.89、3.57、1.52 mg/L。

Abstract: Microwave-assisted ethanol immersion extraction was adopted for extracting flavonoids from the leaf of Baoshan Agastacbe rugose and the extraction technology was optimized with single factor experiment and L₉(3⁴)orthogonal design. With the ascorbic acid as the contrast,its reducing power and scavenging activities of ·OH,DPPH·,ABTS⁺· diammonium salt were discussed.The results showed that the optimum condition of flavonoids extraction:Solid-liquid ratio was 1:60 g/mL,ethanol concentration was 60%,microwave power was 567 W and microwave time was 70 s,the extraction could reach to 6.11%. The flavonoids extracted from the leaf of Agastacbe rugose under these conditions had good ability to scavenge several free radicals, especially ABTS⁺·, the IC₅₀ of ·OH,DPPH· and ABTS⁺· was 281.89,3.57 and 1.52 mg/L.