响应面法优化'凤丹’牡丹花瓣多酚提取工艺

陈刚; 王国霞; 马晓; 陈雅君; 罗青; 陈丽培; 杨玉珍

doi:10.13386/j.issn1002-0306.2018.22.028

响应面法优化'凤丹’牡丹花瓣多酚提取工艺

Optimization of Extraction Technique of Polyphenols from Paeonia suffruticosa ‘Feng Dan’ Petals by Response Surface Methodology

摘要

摘要: 为优化牡丹花瓣多酚提取工艺,以‘凤丹’牡丹花瓣为试验材料,多酚含量为考察指标,研究液料比、超声时间、超声功率、乙醇体积分数四个因素对‘凤丹’牡丹花瓣多酚含量的影响。在单因素实验基础上,通过Box-Behnken实验设计方案优化多酚的最佳提取条件。结果表明,‘凤丹’牡丹花瓣多酚最佳提取工艺条件为:液料比43:1 mL/g,超声时间30 min,超声功率72 W,乙醇体积分数63%。在此条件下,多酚含量为(52.111±1.752)mg/g,优化后的提取工艺对‘凤丹’牡丹花瓣多酚的提取有一定指导意义。

Abstract: To optimize the conditions for the extraction of polyphenols,using Paeonia suffruticosa ‘Feng Dan’ petals as raw material,with polyphenols content as test index to extraction. The effects of the ratio of liquid to solid,ultrasonic time,ultrasonic power and ethanol volume fraction on the polyphenols content were investigated. Experiment factors and their levels were determined by single factor test. Subsequently,the Box-Behnken experimental design was performed to optimize the conditions for polyphenols extraction. The results indicated the optimum conditions for polyphenols extraction were obtained as follows:The ratio of liquid to solid 43:1 mL/g,ultrasonic time 30 min,ultrasonic power 72 W and ethanol volume fraction 63%. Under these conditions,the best polyphenols content was(52.111±1.752)mg/g. The optimized technology applied guiding significance for the ‘Feng Dan’ petals polyphenols extraction.