Abstract:
In order to collect,protect and exploit the yeast resources of traditional fermented dairy products in western pastoral areas,a total of eight samples of pastoral homemade dairy products were collected from the pastoral areas of Tacheng,Xinjiang. Yeast was isolated, purified and identified using the method of cell morphology,physiological and biochemical characterization and 5.8S rDNA sequence homology comparison,and its resistance to high temperature,osmotic pressure and ethanol was investigated. Results showed that,16 strains of
Pichia kudriavzevii,6 strains of
Torulaspora delbrueckii,4 strains of
Metschnikowia pulcherrima,2 strains of
Kluyveromyces marxianus,2 strains of
Saccharomyces cerevisiae,2 strains of
Rhodotorula mucilaginosa,2 strains of
Kluyveromyces lactis were isolated.
Pichia kudriavzevii A2-1 was able to tolerate 42℃ high temperature,2.4% high salt stress,50 g/100 g high glucose stress and 12% alcohol stress;
Kluyveromyces marxianus A2-13 was able to tolerate 42℃ high temperature,2.4% high salt stress,50 g/100 g high glucose stress and 16% alcohol stress;and both strains were able to adapt to the needs of industrial development.
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